Characterization of Freshly Isolated Campylobacter Coli Strains and Suitability of Selective Media for Their Growth

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1988 Mar 1

PMID 3356790

Citations 10

Authors

L K Ng

D E Taylor

M E Stiles

Affiliations

Soon will be listed here.

Abstract

Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology testing. Tetracycline-resistant isolates were obtained from animals with no recorded exposure to antibiotics. A selection of 12 C. coli and 6 C. jejuni strains was used to determine the ability of fresh isolates to grow on a range of selective media. C. coli isolates were inhibited more than C. jejuni on selective media containing antibiotics. The least inhibitory media were Skirrow medium (M. B. Skirrow, Br. Med. J. 2:9-11, 1977) and the charcoal-based media developed by Hutchinson and Bolton (D. N. Hutchinson and F. J. Bolton, J. Clin. Pathol. 37:956-957, 1984) and Karmali et al. (M. A. Karmali, A. E. Simon, M. Roscoe, P. C. Fleming, S. S. Smith, and J. Lane, J. Clin. Microbiol. 23:456-459, 1986). The plasmid contents of the isolates did not appear to be related to their sensitivity to growth on selective (antibiotic-containing) media. The study indicates that selective media used to detect Campylobacter spp. could select against the isolation of C. coli.

Citing Articles

Culture-Based Standard Methods for the Isolation of spp. in Food and Water.

Neyaz L, Arafa S, Alsulami F, Ashi H, Elbanna K, Abulreesh H Pol J Microbiol. 2024; 73(4):433-454.

PMID: 39670639 PMC: 11639288. DOI: 10.33073/pjm-2024-046.

Infant diarrheal disease in rhesus macaques impedes microbiome maturation and is linked to uncultured Campylobacter species.

Rhoades N, Cinco I, Hendrickson S, Prongay K, Haertel A, Flores G Commun Biol. 2024; 7(1):37.

PMID: 38182754 PMC: 10770169. DOI: 10.1038/s42003-023-05695-0.

Contribution of the multidrug efflux transporter CmeABC to antibiotic resistance in different Campylobacter species.

Guo B, Lin J, Reynolds D, Zhang Q Foodborne Pathog Dis. 2009; 7(1):77-83.

PMID: 19785541 PMC: 3145173. DOI: 10.1089/fpd.2009.0354.

Comparison of loop-mediated isothermal amplification assay and conventional culture methods for detection of Campylobacter jejuni and Campylobacter coli in naturally contaminated chicken meat samples.

Yamazaki W, Taguchi M, Kawai T, Kawatsu K, Sakata J, Inoue K Appl Environ Microbiol. 2009; 75(6):1597-603.

PMID: 19139242 PMC: 2655443. DOI: 10.1128/AEM.02004-08.

Campylobacter curvus-associated hepatic abscesses: a case report.

Wetsch N, Somani K, Tyrrell G, Gebhart C, Bailey R, Taylor D J Clin Microbiol. 2006; 44(5):1909-11.

PMID: 16672438 PMC: 1479193. DOI: 10.1128/JCM.44.5.1909-1911.2006.

References

Roop 2nd R, Smibert R, Krieg N . Improved biotyping schemes for Campylobacter jejuni and Campylobacter coli. J Clin Microbiol. 1984; 20(5):990-2. PMC: 271491. DOI: 10.1128/jcm.20.5.990-992.1984. View

Gebhart C, Edmonds P, Ward G, Kurtz H, Brenner D . "Campylobacter hyointestinalis" sp. nov.: a new species of Campylobacter found in the intestines of pigs and other animals. J Clin Microbiol. 1985; 21(5):715-20. PMC: 271765. DOI: 10.1128/jcm.21.5.715-720.1985. View

Ng L, Stiles M, Taylor D . Inhibition of Campylobacter coli and Campylobacter jejuni by antibiotics used in selective growth media. J Clin Microbiol. 1985; 22(4):510-4. PMC: 268456. DOI: 10.1128/jcm.22.4.510-514.1985. View

Megraud F, Elharrif Z . Isolation of Campylobacter species by filtration. Eur J Clin Microbiol. 1985; 4(4):437-8. DOI: 10.1007/BF02148709. View

DEKEYSER P, Butzler J, Sternon J . Acute enteritis due to related vibrio: first positive stool cultures. J Infect Dis. 1972; 125(4):390-2. DOI: 10.1093/infdis/125.4.390. View

Lauwers S, De Boeck M, Butzler J . Campylobacter enteritis in Brussels. Lancet. 1978; 1(8064):604-5. DOI: 10.1016/s0140-6736(78)91045-0. View

Rosef O, Gondrosen B, Kapperud G, Underdal B . Isolation and characterization of Campylobacter jejuni and Campylobacter coli from domestic and wild mammals in Norway. Appl Environ Microbiol. 1983; 46(4):855-9. PMC: 239479. DOI: 10.1128/aem.46.4.855-859.1983. View

Goossens H, De Boeck M, Butzler J . A new selective medium for the isolation of Campylobacter jejuni from human faeces. Eur J Clin Microbiol. 1983; 2(4):389-93. DOI: 10.1007/BF02019476. View

Ng L, Stiles M, Taylor D . Classification of Campylobacter strains using DNA probes. Mol Cell Probes. 1987; 1(3):233-43. DOI: 10.1016/0890-8508(87)90036-3. View

10.

Waterman S, Park R, Bramley A . A search for the source of Campylobacter jejuni in milk. J Hyg (Lond). 1984; 93(2):333-7. PMC: 2129423. DOI: 10.1017/s0022172400064871. View

11.

Taylor D, Ng L, Lior H . Susceptibility of Campylobacter species to nalidixic acid, enoxacin, and other DNA gyrase inhibitors. Antimicrob Agents Chemother. 1985; 28(5):708-10. PMC: 176366. DOI: 10.1128/AAC.28.5.708. View

12.

Davies J, Smith D . Plasmid-determined resistance to antimicrobial agents. Annu Rev Microbiol. 1978; 32:469-518. DOI: 10.1146/annurev.mi.32.100178.002345. View

13.

Lewis B . Phosphatase production by staphylococci--a comparison of two methods. J Med Lab Technol. 1961; 18:112-3. View

14.

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

15.

SKIRROW M . Campylobacter enteritis: a "new" disease. Br Med J. 1977; 2(6078):9-11. PMC: 1631297. DOI: 10.1136/bmj.2.6078.9. View

16.

Karmali M, Simor A, Roscoe M, Fleming P, Smith S, Lane J . Evaluation of a blood-free, charcoal-based, selective medium for the isolation of Campylobacter organisms from feces. J Clin Microbiol. 1986; 23(3):456-9. PMC: 268673. DOI: 10.1128/jcm.23.3.456-459.1986. View

17.

Hutchinson D, Bolton F . Improved blood free selective medium for the isolation of campylobacter jejuni from faecal specimens. J Clin Pathol. 1984; 37(8):956-7. PMC: 498901. DOI: 10.1136/jcp.37.8.956-b. View

18.

Patton C, Mitchell S, Potter M, Kaufmann A . Comparison of selective media for primary isolation of Campylobacter fetus subsp. jejuni. J Clin Microbiol. 1981; 13(2):326-30. PMC: 273785. DOI: 10.1128/jcm.13.2.326-330.1981. View

19.

Blaser M, Berkowitz I, LaForce F, CRAVENS J, Reller L, Wang W . Campylobacter enteritis: clinical and epidemiologic features. Ann Intern Med. 1979; 91(2):179-85. DOI: 10.7326/0003-4819-91-2-179. View

20.

MARMUR J, Doty P . Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol. 1962; 5:109-18. DOI: 10.1016/s0022-2836(62)80066-7. View