The Cyclic Gene Hes1 Contributes to Diverse Differentiation Responses of Embryonic Stem Cells

Overview

Journal Genes Dev

Specialty Molecular Biology

Date 2009 Aug 18

PMID 19684110

Citations 119

Authors

Taeko Kobayashi

Hiroaki Mizuno

Itaru Imayoshi

Chikara Furusawa

Katsuhiko Shirahige

Ryoichiro Kageyama

Affiliations

Soon will be listed here.

Abstract

Stem cells do not all respond the same way, but the mechanisms underlying this heterogeneity are not well understood. Here, we found that expression of Hes1 and its downstream genes oscillate in mouse embryonic stem (ES) cells. Those expressing low and high levels of Hes1 tended to differentiate into neural and mesodermal cells, respectively. Furthermore, inactivation of Hes1 facilitated neural differentiation more uniformly at earlier time. Thus, Hes1-null ES cells display less heterogeneity in both the differentiation timing and fate choice, suggesting that the cyclic gene Hes1 contributes to heterogeneous responses of ES cells even under the same environmental conditions.

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