Rye B Chromosomes Are Weakly Transcribed and Might Alter the Transcriptional Activity of A Chromosome Sequences

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 2009 Jul 4

PMID 19575213

Citations 36

Authors

Mariana Carchilan

Katrin Kumke

Sabine Mikolajewski

Andreas Houben

Affiliations

Soon will be listed here.

Abstract

B chromosomes (Bs) are dispensable components of the genomes of numerous species. To test whether the transcriptome of a host is influenced by Bs, we looked for differences in expression in response to additional Bs. Comparative complementary DNA amplified fragment length polymorphism experiments resulted in the identification of 16 putative B-chromosome-associated transcripts. This comprises 0.7% of the total transcript number and indicates a low activity of Bs. We also provide evidence that B chromosome influences in trans the transcription of A chromosome sequences. The B-specific transcribed sequences B1334, B8149, and B2465 belong to high-copy families with similarity to mobile elements. For all analyzed B-chromosome-derived transcripts, similar A chromosome-encoded sequences were found which supports an A-derived origin of rye B chromosomes.

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