Article: The influence of B chromosomes on rDNA organization in rye interphase nuclei

Patterns of rye rDNA organization in interphase nuclei were studied through the use of in situ hybridization in spreads of root meristem cells from plants with and without B chromosomes (Bs). In cells from plants without Bs each rDNA locus is organized as a single perinucleolar knob of condensed chromatin with decondensed chromatin inside the nucleolus. In plants with Bs there is a marked modification of the pattern, found in more than 23% of nuclei, which involves several regions of condensed chromatin interspersed with decondensed chromatin inside the nucleolus. This B-induced alteration in rDNA interphase organization suggests a change in expression of the rRNA genes located on the A chromosomes probably related to the reduction in nuclear RNA observed previously in plants with Bs. The influence of the Bs on the expression of A chromosome genes, through rearrangement of interphase chromatin, could provide the basis of an explanation for some of the known phenotypic effects of B chromosomes in rye.

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References

1.

Manuelidis L, Borden J . Reproducible compartmentalization of individual chromosome domains in human CNS cells revealed by in situ hybridization and three-dimensional reconstruction. Chromosoma. 1988; 96(6):397-410. DOI: 10.1007/BF00303033. View

2.

Leitch A, Mosgoller W, Shi M, Heslop-Harrison J . Different patterns of rDNA organization at interphase in nuclei of wheat and rye. J Cell Sci. 1992; 101 ( Pt 4):751-7. DOI: 10.1242/jcs.101.4.751. View

3.

Schlegel R, Melz G, Mettin D . Rye cytology, cytogenetics and genetics - current status. Theor Appl Genet. 2013; 72(6):721-34. DOI: 10.1007/BF00266535. View

4.

Blunden R, Wilkes T, Forster J, Jimenez M, Sandery M, Karp A . Identification of the E3900 family, a second family of rye B chromosome specific repeated sequences. Genome. 1993; 36(4):706-11. DOI: 10.1139/g93-095. View

5.

Heslop-Harrison J . Gene expression and parental dominance in hybrid plants. Dev Suppl. 1990; :21-8. View

The Influence of B Chromosomes on RDNA Organization in Rye Interphase Nuclei