Single-step Method of RNA Isolation by Acid Guanidinium Thiocyanate-phenol-chloroform Extraction

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1987 Apr 1

PMID 2440339

Citations 13651

Authors

P Chomczynski

N Sacchi

Affiliations

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Abstract

A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described. The method provides a pure preparation of undegraded RNA in high yield and can be completed within 4 h. It is particularly useful for processing large numbers of samples and for isolation of RNA from minute quantities of cells or tissue samples.

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