Solubilization of the Epstein-Barr Virus-determined Nuclear Antigen and Its Characterization As a DNA-binding Protein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1977 Apr 1

PMID 192908

Citations 18

Authors

J Luka

W Siegert

G Klein

Affiliations

Soon will be listed here.

Abstract

The Eptstein-Barr virus (EBV)-determined nuclear antigen (EBNA) was solubilized from isolate nuclei of two EBV-transformed cell lines- Raji and AW-Ramos, by high-salt treatment. Its DNA-binding properties were studied by DNA-cellulose chromatography and a 51Cr release complement fixation assay. EBNA binds to both double-stranded and single-stranded calf thymus DNA, showing a higher affinity to double-stranded DNA. There was no detectable difference in the DNA binding of EBNA prepared from Raji and AW-Ramos cells.

Citing Articles

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No activation of new initiation points for deoxyribonucleic acid replication in BALB/c 3T3 cells transformed by Kirsten sarcoma virus.

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Initiation points for cellular deoxyribonucleic acid replication in human lymphoid cells converted by Epstein-Barr virus.

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Identification of an Epstein-Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoelectrophoresis.

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Two Epstein-Barr viral nuclear neoantigens distinguished by gene transfer, serology, and chromosome binding.

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