T Antigen Binds to Simian Virus 40 DNA at the Origin of Replication

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Apr 1

PMID 165517

Citations 83

Authors

S I Reed

J Ferguson

R W Davis

G R Stark

Affiliations

Soon will be listed here.

Abstract

A technique employing ferritin-conjugated antibody has been developed to visualize specific protein-DNA complexes in the electron microscope and has been used to demonstrate the preferential binding of simian virus 40 (SV40) T antigen at or near the origin of replication of SV40 DNA, 0.67 fractional length clockwise from the EcoRI restriction endonuclease cleavage site. urified covalently closed supercoiled circles of SV40DNA were treated with partially purified T antigen and the complex was stabilized by crosslinking with glutaraldehyde. Hamster antiT antigen gamma-globulin, ferritin-labeled goat anti-hamster gamma-globulin, and glutaraldehyde were then added sequentially. The location of the bound ferritin cores was measured with respect to the EcoRI cleavage site and the orientation of the cores relative to the ends of the DNA was determined with respect to the locations of Escherichia coli DNA unwinding protein, which binds to covalently closed supercoiled SV40 DNA at either of two preferred sites, 0.46 or 0.90 fractional length clockwide from the EcoRI cleavage site.

Citing Articles

Protein encoded by HSV-1 stimulation-related gene 1 (HSRG1) interacts with and inhibits SV40 large T antigen.

Guo H, Cun W, Liu L, Dong S, Wang L, Dong C Cell Prolif. 2006; 39(6):507-18.

PMID: 17109635 PMC: 6496204. DOI: 10.1111/j.1365-2184.2006.00408.x.

Simian virus 40 infection of humans.

Garcea R, Imperiale M J Virol. 2003; 77(9):5039-45.

PMID: 12692206 PMC: 153983. DOI: 10.1128/jvi.77.9.5039-5045.2003.

Simian virus 40 large T antigen stabilizes the TATA-binding protein-TFIIA complex on the TATA element.

Damania B, Lieberman P, Alwine J Mol Cell Biol. 1998; 18(7):3926-35.

PMID: 9632777 PMC: 108977. DOI: 10.1128/MCB.18.7.3926.

A TEF-1-independent mechanism for activation of the simian virus 40 (SV40) late promoter by mutant SV40 large T antigens.

Casaz P, Rice P, Cole C, Hansen U J Virol. 1995; 69(6):3501-9.

PMID: 7745697 PMC: 189063. DOI: 10.1128/JVI.69.6.3501-3509.1995.

Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.

Greenspan D, Carroll R Proc Natl Acad Sci U S A. 1981; 78(1):105-9.

PMID: 6941238 PMC: 318999. DOI: 10.1073/pnas.78.1.105.

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