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Use of Isolated Nuclei in the Indirect Fluorescent-antibody Test for Human Cytomegalovirus Infection: Comparison with Microneutralization, Anticomplement, and Conventional Indirect Fluorescent-antibody Assays

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1978 May 1
PMID 207719
Citations 10
Authors
S Stagno
D W Reynolds
R J Smith
Affiliations
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Abstract

Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antigen on 100 normal human sera was 99, 100, and 98% when compared with microneutralization, anticomplement immunofluorescence, and conventional indirect fluorescent-antibody assays, respectively. Also, 95% of the antibody titers obtained with the nuclear antigen had a perfect correlation with or were within a fourfold-dilution difference of the antibody levels obtained by anticomplement immunofluorescence and the conventional indirect fluorescent-antibody test.

Citing Articles

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Application of a human osteogenic sarcoma cell culture for detection of human cytomegalovirus antibody by immunofluorescence tests.

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Immunofluorescence technique for detection of human cytomegalovirus (HCMV) antibodies against HCMV-induced late antigens with elimination of immunoglobulin G-receptor staining.

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References
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