Thermolabile T (tumor) Antigen from Cells Transformed by a Temperature-sensitive Mutant of Simian Virus 40

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1975 Nov 1

PMID 172906

Citations 33

Authors

D G Tenen

P Baygell

D M Livingston

Affiliations

Soon will be listed here.

Abstract

Partially purified tumor (T) antigen from a strain of Chinese hamster lung cells transformed by wild-type simian virus 40 (SV 40) and either of two temperature-sensitive SV 40 mutants has been studied as a DNA binding protein. The DNA binding activity present in the T-antigen-containing fractions is inhibited by purified hamster anti-T IgG but not by equivalent amounts of nonimmune hamster IgG. T from either wild-type- or tsC219-transformed cells is relatively stable during heating at 44 degrees compared to T prepared from tsA239-transformed cells. These results strongly suggest that T is a product of the SV 40 A gene.

Citing Articles

Complex of simian virus 40 large tumor antigen and 48,000-dalton host tumor antigen.

Greenspan D, Carroll R Proc Natl Acad Sci U S A. 1981; 78(1):105-9.

PMID: 6941238 PMC: 318999. DOI: 10.1073/pnas.78.1.105.

Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.

Montenarh M, Kohler M, Henning R J Virol. 1984; 49(3):658-64.

PMID: 6321754 PMC: 255521. DOI: 10.1128/JVI.49.3.658-664.1984.

Stabilization of the large T protein in temperature-independent (type A) FR 3T3 rat cells transformed with the simian virus 40 tsA30 mutant.

Imbert J, Clertant P, De Bovis B, Planche J, Birg F J Virol. 1983; 47(3):442-51.

PMID: 6312077 PMC: 255285. DOI: 10.1128/JVI.47.3.442-451.1983.

Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.

Rudolph K, Mann K J Virol. 1983; 47(2):276-86.

PMID: 6312066 PMC: 255259. DOI: 10.1128/JVI.47.2.276-286.1983.

Thermolabile in vivo DNA-binding activity associated with a protein encoded by mutants of herpes simplex virus type 1.

Lee C, Knipe D J Virol. 1983; 46(3):909-19.

PMID: 6304350 PMC: 256566. DOI: 10.1128/JVI.46.3.909-919.1983.

References

Kuchino T, Yamaguchi N . Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40. J Virol. 1975; 15(6):1302-7. PMC: 354596. DOI: 10.1128/JVI.15.6.1302-1307.1975. View

Roberts B, Gorecki M, Mulligan R, Danna K, Rozenblatt S, Rich A . Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1975; 72(5):1922-6. PMC: 432660. DOI: 10.1073/pnas.72.5.1922. View

Jessel D, Hudson J, Landau T, Tenen D, Livingston D . Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules. Proc Natl Acad Sci U S A. 1975; 72(5):1960-4. PMC: 432668. DOI: 10.1073/pnas.72.5.1960. View

Osborn M, Weber K . SV40: T antigen, the A function and transformation. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:267-76. DOI: 10.1101/sqb.1974.039.01.035. View

Livingston D, Henderson I, Hudson J . SV40 T antigen: partial purification and properties. Cold Spring Harb Symp Quant Biol. 1975; 39 Pt 1:283-9. DOI: 10.1101/sqb.1974.039.01.037. View

Sambrook J, Sugden B, Keller W, Sharp P . Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A. 1973; 70(12):3711-5. PMC: 427312. DOI: 10.1073/pnas.70.12.3711. View

Hatanaka M, DULBECCO R . Induction of DNA synthesis by SV40. Proc Natl Acad Sci U S A. 1966; 56(2):736-40. PMC: 224434. DOI: 10.1073/pnas.56.2.736. View

Tegtmeyer P, Ozer H . Temperature-sensitive mutants of simian virus 40: infection of permissive cells. J Virol. 1971; 8(4):516-24. PMC: 376225. DOI: 10.1128/JVI.8.4.516-524.1971. View

Tegtmeyer P . Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972; 10(4):591-8. PMC: 356508. DOI: 10.1128/JVI.10.4.591-598.1972. View

10.

Del Villano B, Defendi V . Characterization of the SV40 T antigen. Virology. 1973; 51(1):34-46. DOI: 10.1016/0042-6822(73)90363-2. View

11.

Khoury G, Martin M, Lee T, Danna K, Nathans D . A map of simian virus 40 transcription sites expressed in productively infected cells. J Mol Biol. 1973; 78(2):377-89. DOI: 10.1016/0022-2836(73)90123-x. View

12.

Chou J, Martin R . Complementation analysis of simian virus 40 mutants. J Virol. 1974; 13(5):1101-9. PMC: 355420. DOI: 10.1128/JVI.13.5.1101-1109.1974. View

13.

Chou J, Avila J, Martin R . Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40. J Virol. 1974; 14(1):116-24. PMC: 355484. DOI: 10.1128/JVI.14.1.116-124.1974. View

14.

Lai C, Nathans D . Mapping temperature-sensitive mutants of simian virus 40: rescue of mutants by fragments of viral DNA. Virology. 1974; 60(2):466-75. DOI: 10.1016/0042-6822(74)90340-7. View

15.

Henderson I, Livingston D . Partial purification and characterization of the SV40 T antigen. Cell. 1974; 3(1):65-70. DOI: 10.1016/0092-8674(74)90041-5. View

16.

Carroll R, Hager L, DULBECCO R . Simian virus 40 T antigen binds to DNA. Proc Natl Acad Sci U S A. 1974; 71(9):3754-7. PMC: 433855. DOI: 10.1073/pnas.71.9.3754. View

17.

Wasserman E, Levine L . Quantitative micro-complement fixation and its use in the study of antigenic structure by specific antigen-antibody inhibition. J Immunol. 1961; 87:290-5. View

18.

Black P, Rowe W, Turner H, HUEBNER R . A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS. Proc Natl Acad Sci U S A. 1963; 50:1148-56. PMC: 221288. DOI: 10.1073/pnas.50.6.1148. View

19.

Pope J, Rowe W . DETECTION OF SPECIFIC ANTIGEN IN SV40-TRANSFORMED CELLS BY IMMUNOFLUORESCENCE. J Exp Med. 1964; 120:121-8. PMC: 2137732. DOI: 10.1084/jem.120.2.121. View

20.

Rapp F, Butel J, Feldman L, Kitahara T, Melnick J . DIFFERENTIAL EFFECTS OF INHIBITORS ON THE STEPS LEADING TO THE FORMATION OF SV40 TUMOR AND VIRUS ANTIGENS. J Exp Med. 1965; 121:935-44. PMC: 2138018. DOI: 10.1084/jem.121.6.935. View