Chapter 11. Identification and Analysis of TRNAs That Are Degraded in Saccharomyces Cerevisiae Due to Lack of Modifications

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2009 Feb 14

PMID 19215761

Citations 10

Authors

Irina Chernyakov

Melanie A Baker

Elizabeth J Grayhack

Eric M Phizicky

Affiliations

Soon will be listed here.

Abstract

Mounting evidence shows that tRNA modifications play crucial roles in the maintenance of wild-type levels of several tRNA species. This chapter describes a generalized framework in which to study tRNA turnover in the yeast Saccharomyces cerevisiae as a consequence of a defect in tRNA modification status. It describes several approaches for the identification of tRNA species that are reduced as a consequence of a modification defect, methods for analysis of the rate of tRNA loss and analysis of its aminoacylation, and methods for initial characterization of tRNA turnover. These approaches have been used successfully for several modification defects that result in tRNA turnover.

Citing Articles

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