A Systematic Library for Comprehensive Overexpression Screens in Saccharomyces Cerevisiae

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2008 Feb 5

PMID 18246075

Citations 121

Authors

Grace Marie Jones

Jim Stalker

Sean Humphray

Anthony West

Tony Cox

Jane Rogers

Ian Dunham

Gregory Prelich

Affiliations

Soon will be listed here.

Abstract

Modern genetic analysis requires the development of new resources to systematically explore gene function in vivo. Overexpression screens are a powerful method to investigate genetic pathways, but the goal of routine and comprehensive overexpression screens has been hampered by the lack of systematic libraries. Here we describe the construction of a systematic collection of the Saccharomyces cerevisiae genome in a high-copy vector and its validation in two overexpression screens.

Citing Articles

Aneuploidy of specific chromosomes is beneficial to cells lacking spindle checkpoint protein Bub3.

Gadgil P, Ballew O, Sullivan T, Lacefield S PLoS Genet. 2025; 21(2):e1011576.

PMID: 39903784 PMC: 11819610. DOI: 10.1371/journal.pgen.1011576.

Identification of factors limiting the allotopic production of the Cox2 subunit of yeast cytochrome c oxidase.

Nieto-Panqueva F, Vazquez-Acevedo M, Hamel P, Gonzalez-Halphen D Genetics. 2024; 227(2).

PMID: 38626319 PMC: 11492495. DOI: 10.1093/genetics/iyae058.

RNA cis-regulators are important for Streptococcus pneumoniae in vivo success.

Warrier I, Perry A, Hubbell S, Eichelman M, van Opijnen T, Meyer M PLoS Genet. 2024; 20(3):e1011188.

PMID: 38442125 PMC: 10942264. DOI: 10.1371/journal.pgen.1011188.

Evidence that Xrn1 is in complex with Gcn1, and is required for full levels of eIF2α phosphorylation.

Shanmugam R, Anderson R, Schiemann A, Sattlegger E Biochem J. 2024; 481(7):481-498.

PMID: 38440860 PMC: 11088878. DOI: 10.1042/BCJ20220531.

In vitro reconstitution of chromatin domains shows a role for nucleosome positioning in 3D genome organization.

Oberbeckmann E, Quililan K, Cramer P, Oudelaar A Nat Genet. 2024; 56(3):483-492.

PMID: 38291333 PMC: 10937381. DOI: 10.1038/s41588-023-01649-8.