Purification and Characterization of Cob(II)yrinic Acid A,c-diamide Reductase from Pseudomonas Denitrificans

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1992 Nov 1

PMID 1429467

Citations 10

Authors

F Blanche

L Maton

L Debussche

D THIBAUT

Affiliations

Soon will be listed here.

Abstract

An NADH-dependent flavoenzyme exhibiting cob(II)yrinic acid a,c-diamide reductase activity was purified 6,300-fold to homogeneity from Pseudomonas denitrificans and sequenced at its N terminus. This enzyme of the cobalamin biosynthetic pathway reduced to the Co(I) state all of the Co(II)-corrinoids isolated from this microorganism.

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References

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Crouzet J, Cameron B, Cauchois L, Rigault S, Rouyez M, Blanche F . Nucleotide sequence and genetic analysis of a 13.1-kilobase-pair Pseudomonas denitrificans DNA fragment containing five cob genes and identification of structural genes encoding Cob(I)alamin adenosyltransferase, cobyric acid synthase, and.... J Bacteriol. 1991; 173(19):6074-87. PMC: 208354. DOI: 10.1128/jb.173.19.6074-6087.1991. View

Blanche F, Couder M, Debussche L, THIBAUT D, Cameron B, Crouzet J . Biosynthesis of vitamin B12: stepwise amidation of carboxyl groups b, d, e, and g of cobyrinic acid a,c-diamide is catalyzed by one enzyme in Pseudomonas denitrificans. J Bacteriol. 1991; 173(19):6046-51. PMC: 208350. DOI: 10.1128/jb.173.19.6046-6051.1991. View

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