Functional Phenotyping and Genotyping of Circulating Tumor Cells from Patients with Castration Resistant Prostate Cancer

Overview

Journal Cancer Lett

Specialty Oncology

Date 2009 Jan 24

PMID 19162393

Citations 42

Authors

Pamela L Paris

Yasuko Kobayashi

Qiang Zhao

Wei Zeng

Shivaranjani Sridharan

Tina Fan

Howard L Adler

Emmanuel R Yera

M H Zarrabi

Stanley Zucker

Jeffry Simko

Wen-Tien Chen

Jonathan Rosenberg

Affiliations

Soon will be listed here.

Abstract

Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients' blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.

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