Recent Advances in Methods for Circulating Tumor Cell Detection

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2023 Feb 25

PMID 36835311

Authors

Monika Vidlarova

Alona Rehulkova

Pavel Stejskal

Andrea Prokopova

Hanus Slavik

Marian Hajduch

Josef Srovnal

Affiliations

Soon will be listed here.

Abstract

Circulating tumor cells (CTCs) are released from primary tumors and transported through the body via blood or lymphatic vessels before settling to form micrometastases under suitable conditions. Accordingly, several studies have identified CTCs as a negative prognostic factor for survival in many types of cancer. CTCs also reflect the current heterogeneity and genetic and biological state of tumors; so, their study can provide valuable insights into tumor progression, cell senescence, and cancer dormancy. Diverse methods with differing specificity, utility, costs, and sensitivity have been developed for isolating and characterizing CTCs. Additionally, novel techniques with the potential to overcome the limitations of existing ones are being developed. This primary literature review describes the current and emerging methods for enriching, detecting, isolating, and characterizing CTCs.

Citing Articles

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A revolutionary era in advancing precision immuno-oncology; role of circulating tumor cells.

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Liquid biopsy for Renal Cell Carcinoma: A comprehensive review of techniques, applications, and future prospects.

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References

Neoh K, Hassan A, Chen A, Sun Y, Liu P, Xu K . Rethinking liquid biopsy: Microfluidic assays for mobile tumor cells in human body fluids. Biomaterials. 2017; 150:112-124. DOI: 10.1016/j.biomaterials.2017.10.006. View

Sharma S, Zhuang R, Long M, Pavlovic M, Kang Y, Ilyas A . Circulating tumor cell isolation, culture, and downstream molecular analysis. Biotechnol Adv. 2018; 36(4):1063-1078. PMC: 5971144. DOI: 10.1016/j.biotechadv.2018.03.007. View

Nasiri R, Shamloo A, Ahadian S, Amirifar L, Akbari J, Goudie M . Microfluidic-Based Approaches in Targeted Cell/Particle Separation Based on Physical Properties: Fundamentals and Applications. Small. 2020; 16(29):e2000171. DOI: 10.1002/smll.202000171. View

Lu R, Chen Q, Liu X, Shen S, Pan Z, Shi C . Detection of circulating stage III-IV gastric cancer tumor cells based on isolation by size of epithelial tumor: using the circulating tumor cell biopsy technology. Transl Cancer Res. 2022; 8(4):1342-1350. PMC: 8798136. DOI: 10.21037/tcr.2019.07.32. View

Baslan T, Hicks J . Unravelling biology and shifting paradigms in cancer with single-cell sequencing. Nat Rev Cancer. 2017; 17(9):557-569. DOI: 10.1038/nrc.2017.58. View

Konigsberg R, Gneist M, Jahn-Kuch D, Pfeiler G, Hager G, Hudec M . Circulating tumor cells in metastatic colorectal cancer: efficacy and feasibility of different enrichment methods. Cancer Lett. 2010; 293(1):117-23. DOI: 10.1016/j.canlet.2010.01.003. View

Yang C, Xia B, Jin W, Lou G . Circulating tumor cells in precision oncology: clinical applications in liquid biopsy and 3D organoid model. Cancer Cell Int. 2019; 19:341. PMC: 6918690. DOI: 10.1186/s12935-019-1067-8. View

Jia F, Wang Y, Fang Z, Dong J, Shi F, Zhang W . Novel Peptide-Based Magnetic Nanoparticle for Mesenchymal Circulating Tumor Cells Detection. Anal Chem. 2021; 93(14):5670-5675. DOI: 10.1021/acs.analchem.1c00577. View

Gorges T, Stein A, Quidde J, Hauch S, Rock K, Riethdorf S . Improved Detection of Circulating Tumor Cells in Metastatic Colorectal Cancer by the Combination of the CellSearch® System and the AdnaTest®. PLoS One. 2016; 11(5):e0155126. PMC: 4868337. DOI: 10.1371/journal.pone.0155126. View

10.

Gabriel M, Rodriguez Calleja L, Chalopin A, Ory B, Heymann D . Circulating Tumor Cells: A Review of Non-EpCAM-Based Approaches for Cell Enrichment and Isolation. Clin Chem. 2016; 62(4):571-81. DOI: 10.1373/clinchem.2015.249706. View

11.

Eslami-S Z, Cortes-Hernandez L, Alix-Panabieres C . Epithelial Cell Adhesion Molecule: An Anchor to Isolate Clinically Relevant Circulating Tumor Cells. Cells. 2020; 9(8). PMC: 7464831. DOI: 10.3390/cells9081836. View

12.

Wang W, Peng J, Li X, Wang A, Bie Y, Shi L . Survival Mechanisms and Influence Factors of Circulating Tumor Cells. Biomed Res Int. 2018; 2018:6304701. PMC: 6236925. DOI: 10.1155/2018/6304701. View

13.

Zheng S, Lin H, Liu J, Balic M, Datar R, Cote R . Membrane microfilter device for selective capture, electrolysis and genomic analysis of human circulating tumor cells. J Chromatogr A. 2007; 1162(2):154-61. DOI: 10.1016/j.chroma.2007.05.064. View

14.

Eibl R, Schneemann M . Liquid biopsy and glioblastoma. Explor Target Antitumor Ther. 2023; 4(1):28-41. PMC: 10017188. DOI: 10.37349/etat.2023.00121. View

15.

Alix-Panabieres C, Riethdorf S, Pantel K . Circulating tumor cells and bone marrow micrometastasis. Clin Cancer Res. 2008; 14(16):5013-21. DOI: 10.1158/1078-0432.CCR-07-5125. View

16.

Abouleila Y, Onidani K, Ali A, Shoji H, Kawai T, Lim C . Live single cell mass spectrometry reveals cancer-specific metabolic profiles of circulating tumor cells. Cancer Sci. 2018; 110(2):697-706. PMC: 6361580. DOI: 10.1111/cas.13915. View

17.

Liu Y, Li R, Zhang L, Guo S . Nanomaterial-Based Immunocapture Platforms for the Recognition, Isolation, and Detection of Circulating Tumor Cells. Front Bioeng Biotechnol. 2022; 10:850241. PMC: 8964261. DOI: 10.3389/fbioe.2022.850241. View

18.

Kitz J, Goodale D, Postenka C, Lowes L, Allan A . EMT-independent detection of circulating tumor cells in human blood samples and pre-clinical mouse models of metastasis. Clin Exp Metastasis. 2021; 38(1):97-108. PMC: 7882592. DOI: 10.1007/s10585-020-10070-y. View

19.

Mishra A, Dubash T, Edd J, Jewett M, Garre S, Karabacak N . Ultrahigh-throughput magnetic sorting of large blood volumes for epitope-agnostic isolation of circulating tumor cells. Proc Natl Acad Sci U S A. 2020; 117(29):16839-16847. PMC: 7382214. DOI: 10.1073/pnas.2006388117. View

20.

Biezuner T, Raz O, Amir S, Milo L, Adar R, Fried Y . Comparison of seven single cell whole genome amplification commercial kits using targeted sequencing. Sci Rep. 2021; 11(1):17171. PMC: 8387353. DOI: 10.1038/s41598-021-96045-9. View