Posttranscriptional Crossregulation Between Drosha and DGCR8

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2009 Jan 13

PMID 19135890

Citations 243

Authors

Jinju Han

Jakob S Pedersen

S Chul Kwon

Cassandra D Belair

Young-Kook Kim

Kyu-Hyeon Yeom

Woo-Young Yang

David Haussler

Robert Blelloch

V Narry Kim

Affiliations

Soon will be listed here.

Abstract

The Drosha-DGCR8 complex, also known as Microprocessor, is essential for microRNA (miRNA) maturation. Drosha functions as the catalytic subunit, while DGCR8 (also known as Pasha) recognizes the RNA substrate. Although the action mechanism of this complex has been intensively studied, it remains unclear how Drosha and DGCR8 are regulated and if these proteins have any additional role(s) apart from miRNA processing. Here, we report that Drosha and DGCR8 regulate each other posttranscriptionally. The Drosha-DGCR8 complex cleaves the hairpin structures embedded in the DGCR8 mRNA and thereby destabilizes the mRNA. We further find that DGCR8 stabilizes the Drosha protein via protein-protein interaction. This crossregulation between Drosha and DGCR8 may contribute to the homeostatic control of miRNA biogenesis. Furthermore, microarray analyses suggest that a number of mRNAs may be downregulated in a Microprocessor-dependent, miRNA-independent manner. Our study reveals a previously unsuspected function of Microprocessor in mRNA stability control.

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