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Field-based Angle-resolved Light-scattering Study of Single Live Cells

Overview
Journal Opt Lett
Specialty Ophthalmology
Date 2008 Jul 17
PMID 18628809
Citations 14
Authors
Wonshik Choi
Chung-Chieh Yu
Christopher Fang-Yen
Kamran Badizadegan
Ramachandra R Dasari
Michael S Feld
Affiliations
Soon will be listed here.
Abstract

We perform field-based angle-resolved light-scattering measurements from single live cells. We use a laser interferometer to acquire phase and amplitude images of cells at the image plane. The angular scattering spectrum is calculated from the Fourier transform of the field transmitted through the cells. A concurrent 3D refractive index distribution of the same cells is measured using tomographic phase microscopy. By measuring transient increases in light scattering by single cells during exposure to acetic acid, we correlate the scattering properties of single cells with their refractive index distributions and show that results are in good agreement with a model based on the Born approximation.

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References
1.
Choi W, Fang-Yen C, Badizadegan K, Dasari R, Feld M . Extended depth of focus in tomographic phase microscopy using a propagation algorithm. Opt Lett. 2008; 33(2):171-3. PMC: 2851251. DOI: 10.1364/ol.33.000171. View
2.
Wilson J, Foster T . Characterization of lysosomal contribution to whole-cell light scattering by organelle ablation. J Biomed Opt. 2007; 12(3):030503. DOI: 10.1117/1.2743971. View
3.
Sokolov K, Drezek R, Gossage K, Richards-Kortum R . Reflectance spectroscopy with polarized light: is it sensitive to cellular and nuclear morphology. Opt Express. 2009; 5(13):302-17. DOI: 10.1364/oe.5.000302. View
4.
Wu T, Qu J, Xu M . Unified Mie and fractal scattering by biological cells and subcellular structures. Opt Lett. 2007; 32(16):2324-6. DOI: 10.1364/ol.32.002324. View
5.
Choi W, Fang-Yen C, Badizadegan K, Oh S, Lue N, Dasari R . Tomographic phase microscopy. Nat Methods. 2007; 4(9):717-9. DOI: 10.1038/nmeth1078. View