Refractive Index Maps and Membrane Dynamics of Human Red Blood Cells Parasitized by Plasmodium Falciparum

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2008 Sep 6

PMID 18772382

Citations 213

Authors

YongKeun Park

Monica Diez-Silva

Gabriel Popescu

George Lykotrafitis

Wonshik Choi

Michael S Feld

Subra Suresh

Affiliations

Soon will be listed here.

Abstract

Parasitization by malaria-inducing Plasmodium falciparum leads to structural, biochemical, and mechanical modifications to the host red blood cells (RBCs). To study these modifications, we investigate two intrinsic indicators: the refractive index and membrane fluctuations in P. falciparum-invaded human RBCs (Pf-RBCs). We report experimental connections between these intrinsic indicators and pathological states. By employing two noninvasive optical techniques, tomographic phase microscopy and diffraction phase microscopy, we extract three-dimensional maps of refractive index and nanoscale cell membrane fluctuations in isolated RBCs. Our systematic experiments cover all intraerythrocytic stages of parasite development under physiological and febrile temperatures. These findings offer potential, and sufficiently general, avenues for identifying, through cell membrane dynamics, pathological states that cause or accompany human diseases.

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