Ultrabroadband Optical Diffraction Tomography

Overview

Journal ACS Photonics

Publisher American Chemical Society

Date 2024 Sep 23

PMID 39310293

Authors

Martin Hormann

Franco V A Camargo

Niek F van Hulst

Giulio Cerullo

Matz Liebel

Affiliations

Soon will be listed here.

Abstract

Optical diffraction tomography (ODT) is a powerful noninvasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping-based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of nanoparticles within cellular structures. Our work represents a crucial step toward time and spectrally resolved tomography of complex 3D structures with implications for life and materials science applications.

Citing Articles

Photopic flicker optoretinography captures the light-driven length modulation of photoreceptors during phototransduction.

Tomczewski S, Curatolo A, Foik A, Wegrzyn P, Balamut B, Wielgo M Proc Natl Acad Sci U S A. 2025; 122(7):e2421722122.

PMID: 39946535 PMC: 11848411. DOI: 10.1073/pnas.2421722122.

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