Batf3 Deficiency Reveals a Critical Role for CD8alpha+ Dendritic Cells in Cytotoxic T Cell Immunity

Overview

Journal Science

Specialty Science

Date 2008 Nov 15

PMID 19008445

Citations 1117

Authors

Kai Hildner

Brian T Edelson

Whitney E Purtha

Mark Diamond

Hirokazu Matsushita

Masako Kohyama

Boris Calderon

Barbara U Schraml

Emil R Unanue

Michael S Diamond

Robert D Schreiber

Theresa L Murphy

Kenneth M Murphy

Affiliations

Soon will be listed here.

Abstract

Although in vitro observations suggest that cross-presentation of antigens is mediated primarily by CD8alpha+ dendritic cells, in vivo analysis has been hampered by the lack of systems that selectively eliminate this cell lineage. We show that deletion of the transcription factor Batf3 ablated development of CD8alpha+ dendritic cells, allowing us to examine their role in immunity in vivo. Dendritic cells from Batf3-/- mice were defective in cross-presentation, and Batf3-/- mice lacked virus-specific CD8+ T cell responses to West Nile virus. Importantly, rejection of highly immunogenic syngeneic tumors was impaired in Batf3-/- mice. These results suggest an important role for CD8alpha+ dendritic cells and cross-presentation in responses to viruses and in tumor rejection.

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