BSKs Mediate Signal Transduction from the Receptor Kinase BRI1 in Arabidopsis

Overview

Journal Science

Specialty Science

Date 2008 Jul 26

PMID 18653891

Citations 293

Authors

Wenqiang Tang

Tae-Wuk Kim

Juan A Oses-Prieto

Yu Sun

Zhiping Deng

Shengwei Zhu

Ruiju Wang

Alma L Burlingame

Zhi-Yong Wang

Affiliations

Soon will be listed here.

Abstract

Brassinosteroids (BRs) bind to the extracellular domain of the receptor kinase BRI1 to activate a signal transduction cascade that regulates nuclear gene expression and plant development. Many components of the BR signaling pathway have been identified and studied in detail. However, the substrate of BRI1 kinase that transduces the signal to downstream components remains unknown. Proteomic studies of plasma membrane proteins lead to the identification of three homologous BR-signaling kinases (BSK1, BSK2, and BSK3). The BSKs are phosphorylated by BRI1 in vitro and interact with BRI1 in vivo. Genetic and transgenic studies demonstrate that the BSKs represent a small family of kinases that activate BR signaling downstream of BRI1. These results demonstrate that BSKs are the substrates of BRI1 kinase that activate downstream BR signal transduction.

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References

Wang X, Goshe M, Soderblom E, Phinney B, Kuchar J, Li J . Identification and functional analysis of in vivo phosphorylation sites of the Arabidopsis BRASSINOSTEROID-INSENSITIVE1 receptor kinase. Plant Cell. 2005; 17(6):1685-703. PMC: 1143070. DOI: 10.1105/tpc.105.031393. View

Wang X, Chory J . Brassinosteroids regulate dissociation of BKI1, a negative regulator of BRI1 signaling, from the plasma membrane. Science. 2006; 313(5790):1118-22. DOI: 10.1126/science.1127593. View

Tang W, Deng Z, Oses-Prieto J, Suzuki N, Zhu S, Zhang X . Proteomics studies of brassinosteroid signal transduction using prefractionation and two-dimensional DIGE. Mol Cell Proteomics. 2008; 7(4):728-38. PMC: 2401332. DOI: 10.1074/mcp.M700358-MCP200. View

Vert G, Nemhauser J, Geldner N, Hong F, Chory J . Molecular mechanisms of steroid hormone signaling in plants. Annu Rev Cell Dev Biol. 2005; 21:177-201. DOI: 10.1146/annurev.cellbio.21.090704.151241. View

Gampala S, Kim T, He J, Tang W, Deng Z, Bai M . An essential role for 14-3-3 proteins in brassinosteroid signal transduction in Arabidopsis. Dev Cell. 2007; 13(2):177-89. PMC: 2000337. DOI: 10.1016/j.devcel.2007.06.009. View

Li J, Wen J, Lease K, Doke J, Tax F, Walker J . BAK1, an Arabidopsis LRR receptor-like protein kinase, interacts with BRI1 and modulates brassinosteroid signaling. Cell. 2002; 110(2):213-22. DOI: 10.1016/s0092-8674(02)00812-7. View

Nam K, Li J . BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell. 2002; 110(2):203-12. DOI: 10.1016/s0092-8674(02)00814-0. View

Wang Z, Nakano T, Gendron J, He J, Chen M, Vafeados D . Nuclear-localized BZR1 mediates brassinosteroid-induced growth and feedback suppression of brassinosteroid biosynthesis. Dev Cell. 2002; 2(4):505-13. DOI: 10.1016/s1534-5807(02)00153-3. View

Heese A, Hann D, Gimenez-Ibanez S, Jones A, He K, Li J . The receptor-like kinase SERK3/BAK1 is a central regulator of innate immunity in plants. Proc Natl Acad Sci U S A. 2007; 104(29):12217-22. PMC: 1924592. DOI: 10.1073/pnas.0705306104. View

10.

Gendron J, Wang Z . Multiple mechanisms modulate brassinosteroid signaling. Curr Opin Plant Biol. 2007; 10(5):436-41. PMC: 2093957. DOI: 10.1016/j.pbi.2007.08.015. View

11.

Wang Z, Seto H, Fujioka S, Yoshida S, Chory J . BRI1 is a critical component of a plasma-membrane receptor for plant steroids. Nature. 2001; 410(6826):380-3. DOI: 10.1038/35066597. View

12.

Vert G, Chory J . Downstream nuclear events in brassinosteroid signalling. Nature. 2006; 441(7089):96-100. DOI: 10.1038/nature04681. View

13.

Shiu S, Karlowski W, Pan R, Tzeng Y, Mayer K, Li W . Comparative analysis of the receptor-like kinase family in Arabidopsis and rice. Plant Cell. 2004; 16(5):1220-34. PMC: 423211. DOI: 10.1105/tpc.020834. View

14.

Kemmerling B, Schwedt A, Rodriguez P, Mazzotta S, Frank M, Qamar S . The BRI1-associated kinase 1, BAK1, has a brassinolide-independent role in plant cell-death control. Curr Biol. 2007; 17(13):1116-22. DOI: 10.1016/j.cub.2007.05.046. View

15.

He J, Gendron J, Sun Y, Gampala S, Gendron N, Sun C . BZR1 is a transcriptional repressor with dual roles in brassinosteroid homeostasis and growth responses. Science. 2005; 307(5715):1634-8. PMC: 2925132. DOI: 10.1126/science.1107580. View

16.

Chinchilla D, Zipfel C, Robatzek S, Kemmerling B, Nurnberger T, Jones J . A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature. 2007; 448(7152):497-500. DOI: 10.1038/nature05999. View

17.

Niittyla T, Fuglsang A, Palmgren M, Frommer W, Schulze W . Temporal analysis of sucrose-induced phosphorylation changes in plasma membrane proteins of Arabidopsis. Mol Cell Proteomics. 2007; 6(10):1711-26. DOI: 10.1074/mcp.M700164-MCP200. View

18.

Yin Y, Vafeados D, Tao Y, Yoshida S, Asami T, Chory J . A new class of transcription factors mediates brassinosteroid-regulated gene expression in Arabidopsis. Cell. 2005; 120(2):249-59. DOI: 10.1016/j.cell.2004.11.044. View

19.

Alonso J, Stepanova A, Leisse T, Kim C, Chen H, Shinn P . Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science. 2003; 301(5633):653-7. DOI: 10.1126/science.1086391. View

20.

He K, Gou X, Yuan T, Lin H, Asami T, Yoshida S . BAK1 and BKK1 regulate brassinosteroid-dependent growth and brassinosteroid-independent cell-death pathways. Curr Biol. 2007; 17(13):1109-15. DOI: 10.1016/j.cub.2007.05.036. View