Physiological and Differential Protein Expression Analyses of the Calcium Stress Response in the Rhizome

Overview

Journal Heliyon

Specialty Social Sciences

Date 2024 Oct 10

PMID 39386768

Authors

Yilin Wu

Hui Li

Shanshan Ma

Hongna Ma

Longyan Tan

Affiliations

Soon will be listed here.

Abstract

High concentration Ca in karst soil is harmful to agriculture. Some dominant plants can adapt well to karst soil, but how Ca affect plant is unknown. is a Ca-tolerant fern and its dry rhizome is a common Chinese medicine of Miao nationality in Guizhou, China. This study analyzed the physiological and proteomic characteristics of the rhizome of . under calcium stress. Physiological results indicated that calcium stress may lead to osmotic stress. Proteomic results showed that 147 differentially expressed proteins (96 increased, 51decreased) were identified under calcium stress, and these proteins mainly involved in signal transduction, protein translation, material transport, antioxidant defense and secondary metabolism. This study will lay a foundation for studying the calcium adaptation mechanism of at the molecular level.

References

Carafoli E, Santella L, Branca D, Brini M . Generation, control, and processing of cellular calcium signals. Crit Rev Biochem Mol Biol. 2001; 36(2):107-260. DOI: 10.1080/20014091074183. View

Merlot S, Leonhardt N, Fenzi F, Valon C, Costa M, Piette L . Constitutive activation of a plasma membrane H(+)-ATPase prevents abscisic acid-mediated stomatal closure. EMBO J. 2007; 26(13):3216-26. PMC: 1914098. DOI: 10.1038/sj.emboj.7601750. View

Ho S, Huang L, Lu C, He S, Wang C, Yu S . Sugar starvation- and GA-inducible calcium-dependent protein kinase 1 feedback regulates GA biosynthesis and activates a 14-3-3 protein to confer drought tolerance in rice seedlings. Plant Mol Biol. 2013; 81(4-5):347-61. DOI: 10.1007/s11103-012-0006-z. View

Kumar K, Sinha A . Overexpression of constitutively active mitogen activated protein kinase kinase 6 enhances tolerance to salt stress in rice. Rice (N Y). 2013; 6(1):25. PMC: 4883705. DOI: 10.1186/1939-8433-6-25. View

Fiehn O, Kopka J, Dormann P, Altmann T, Trethewey R, Willmitzer L . Metabolite profiling for plant functional genomics. Nat Biotechnol. 2000; 18(11):1157-61. DOI: 10.1038/81137. View

Pandian B, Sathishraj R, Djanaguiraman M, Prasad P, Jugulam M . Role of Cytochrome P450 Enzymes in Plant Stress Response. Antioxidants (Basel). 2020; 9(5). PMC: 7278705. DOI: 10.3390/antiox9050454. View

Jarrett H, Brown C, Black C, Cormier M . Evidence that calmodulin is in the chloroplast of peas and serves a regulatory role in photosynthesis. J Biol Chem. 1982; 257(22):13795-804. View

BARRIEU , CHAUMONT , Chrispeels . High expression of the tonoplast aquaporin ZmTIP1 in epidermal and conducting tissues of maize . Plant Physiol. 1998; 117(4):1153-63. PMC: 34879. DOI: 10.1104/pp.117.4.1153. View

Wisniewski J, Zougman A, Mann M . Combination of FASP and StageTip-based fractionation allows in-depth analysis of the hippocampal membrane proteome. J Proteome Res. 2009; 8(12):5674-8. DOI: 10.1021/pr900748n. View

10.

Damke H, Baba T, Warnock D, Schmid S . Induction of mutant dynamin specifically blocks endocytic coated vesicle formation. J Cell Biol. 1994; 127(4):915-34. PMC: 2200053. DOI: 10.1083/jcb.127.4.915. View

11.

Jiang N, Doseff A, Grotewold E . Flavones: From Biosynthesis to Health Benefits. Plants (Basel). 2016; 5(2). PMC: 4931407. DOI: 10.3390/plants5020027. View

12.

Heckwolf M, Pater D, Hanson D, Kaldenhoff R . The Arabidopsis thaliana aquaporin AtPIP1;2 is a physiologically relevant CO₂ transport facilitator. Plant J. 2011; 67(5):795-804. DOI: 10.1111/j.1365-313X.2011.04634.x. View

13.

Maurel C . AQUAPORINS AND WATER PERMEABILITY OF PLANT MEMBRANES. Annu Rev Plant Physiol Plant Mol Biol. 1997; 48:399-429. DOI: 10.1146/annurev.arplant.48.1.399. View

14.

Tang W, Kim T, Oses-Prieto J, Sun Y, Deng Z, Zhu S . BSKs mediate signal transduction from the receptor kinase BRI1 in Arabidopsis. Science. 2008; 321(5888):557-60. PMC: 2730546. DOI: 10.1126/science.1156973. View

15.

Upadhyay S . CPK12 and Ca-mediated hypoxia signaling. Plant Signal Behav. 2023; 18(1):2273593. PMC: 10761129. DOI: 10.1080/15592324.2023.2273593. View

16.

Nijtmans L, de Jong L, Artal Sanz M, Coates P, Berden J, Back J . Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins. EMBO J. 2000; 19(11):2444-51. PMC: 212747. DOI: 10.1093/emboj/19.11.2444. View

17.

Batelli G, Verslues P, Agius F, Qiu Q, Fujii H, Pan S . SOS2 promotes salt tolerance in part by interacting with the vacuolar H+-ATPase and upregulating its transport activity. Mol Cell Biol. 2007; 27(22):7781-90. PMC: 2169139. DOI: 10.1128/MCB.00430-07. View

18.

Mori I, Rhee J, Shibasaka M, Sasano S, Kaneko T, Horie T . CO2 transport by PIP2 aquaporins of barley. Plant Cell Physiol. 2014; 55(2):251-7. PMC: 3913445. DOI: 10.1093/pcp/pcu003. View

19.

Ormancey M, Thuleau P, Mazars C, Cotelle V . CDPKs and 14-3-3 Proteins: Emerging Duo in Signaling. Trends Plant Sci. 2017; 22(3):263-272. DOI: 10.1016/j.tplants.2016.11.007. View

20.

Fiehn O . Combining genomics, metabolome analysis, and biochemical modelling to understand metabolic networks. Comp Funct Genomics. 2008; 2(3):155-68. PMC: 2447208. DOI: 10.1002/cfg.82. View