Endocytosis of the Receptor-binding Domain of SARS-CoV Spike Protein Together with Virus Receptor ACE2

Overview

Journal Virus Res

Specialty Microbiology

Date 2008 Jun 17

PMID 18554741

Citations 89

Authors

Shunxin Wang

Feng Guo

Kangtai Liu

Hongliang Wang

Shuan Rao

Peng Yang

Chengyu Jiang

Affiliations

Soon will be listed here.

Abstract

Cell entry of severe acute respiratory syndrome coronavirus (SARS-CoV) is mediated by the viral spike (S) protein. Amino acids 319-510 on the S protein have been mapped as the receptor-binding domain (RBD), which mediates binding to the SARS-CoV receptor angiotensin converting enzyme 2 (ACE2) on SARS-CoV susceptible cells. In this study, we expressed a fusion protein containing the human codon-optimized RBD of the SARS-CoV spike protein linked to the Fc portion of human IgG1 (named RBD-Fc) in HEK293 cells. The RBD-Fc protein was purified by affinity chromatography. The flow cytometry assay showed that the purified RBD-Fc protein could bind to ACE2. We demonstrated that the RBD spike protein alone could be internalized into SARS-CoV susceptible cells together with ACE2. We also showed that the removal of N-glycans from the RBD spike protein did not abolish this phenomenon. Our discoveries may have some implications for the development of the SARS vaccine.

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