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Applications of Direct Detection Device in Transmission Electron Microscopy

Overview
Journal J Struct Biol
Specialties Cell Biology
Molecular Biology
Date 2007 Dec 7
PMID 18054249
Citations 33
Authors
Liang Jin
Anna-Clare Milazzo
Stuart Kleinfelder
Shengdong Li
Philippe Leblanc
Fred Duttweiler
James C Bouwer
Steven T Peltier
Mark H Ellisman
Nguyen-Huu Xuong
Affiliations
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Abstract

A prototype direct detection device (DDD) camera system has shown great promise in improving both the spatial resolution and the signal to noise ratio for electron microscopy at 120-400 keV beam energies (Xuong et al., 2007. Methods in Cell Biology, 79, 721-739). Without the need for a resolution-limiting scintillation screen as in the charge coupled device (CCD), the DDD camera can outperform CCD based systems in terms of spatial resolution, due to its small pixel size (5 microm). In this paper, the modulation transfer function (MTF) of the DDD prototype is measured and compared with the specifications of commercial scientific CCD camera systems. Combining the fast speed of the DDD with image mosaic techniques, fast wide-area imaging is now possible. In this paper, the first large area mosaic image and the first tomography dataset from the DDD camera are presented, along with an image processing algorithm to correct the specimen drift utilizing the fast readout of the DDD system.

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References
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