Multicopy BlaOXA-58 Gene As a Source of High-level Resistance to Carbapenems in Acinetobacter Baumannii

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2007 Apr 18

PMID 17438042

Citations 59

Authors

Alessia Bertini

Laurent Poirel

Sandrine Bernabeu

Daniela Fortini

Laura Villa

Patrice Nordmann

Alessandra Carattoli

Affiliations

Soon will be listed here.

Abstract

The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 in a large University Hospital of Rome, Italy, were investigated. These isolates were related and possessed similar plasmids carrying the carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable levels of resistance to carbapenems. Analysis of sequences surrounding the bla(OXA-58) gene showed genetic variability, with the presence in several isolates of multiple copies of the bla(OXA-58) gene; this extra copy number was likely related to an IS26-mediated transposition or recombination process.

Citing Articles

Characteristics of Oral spp. and Evolution of Plasmid-Mediated Carbapenem Resistance in Bacteremia Patients with Hematological Malignancies.

Li H, Talanaite D, Pan Z, Wang Z, Wang S, Wang H Infect Drug Resist. 2024; 17:4753-4761.

PMID: 39494231 PMC: 11531724. DOI: 10.2147/IDR.S478362.

Exploration of spp. Genome in Search of Antibiotic Resistance.

Bucka-Kolendo J, Kiousi D, Dekowska A, Mikolajczuk-Szczyrba A, Karadedos D, Michael P Int J Mol Sci. 2024; 25(15).

PMID: 39125715 PMC: 11312215. DOI: 10.3390/ijms25158144.

IS and the IS family: versatile resistance gene movers and genome reorganizers.

Harmer C, Hall R Microbiol Mol Biol Rev. 2024; 88(2):e0011922.

PMID: 38436262 PMC: 11332343. DOI: 10.1128/mmbr.00119-22.

In vitro activity of ceftazidime/avibactam, imipenem/relebactam and meropenem/vaborbactam alone or in combination with polymyxin B against carbapenem resistant Acinetobacter baumannii.

Liang R, Wang D, Hu M, Gu Y, Wang M, Hu D J Antibiot (Tokyo). 2023; 76(9):540-547.

PMID: 37217796 DOI: 10.1038/s41429-023-00631-0.

Antimicrobial Resistance in Romania: Updates on Gram-Negative ESCAPE Pathogens in the Clinical, Veterinary, and Aquatic Sectors.

Czobor Barbu I, Gheorghe-Barbu I, Grigore G, Vrancianu C, Chifiriuc M Int J Mol Sci. 2023; 24(9).

PMID: 37175597 PMC: 10178704. DOI: 10.3390/ijms24097892.

References

Riccio M, Franceschini N, Boschi L, Caravelli B, Cornaglia G, Fontana R . Characterization of the metallo-beta-lactamase determinant of Acinetobacter baumannii AC-54/97 reveals the existence of bla(IMP) allelic variants carried by gene cassettes of different phylogeny. Antimicrob Agents Chemother. 2000; 44(5):1229-35. PMC: 89849. DOI: 10.1128/AAC.44.5.1229-1235.2000. View

del Mar Tomas M, Cartelle M, Pertega S, Beceiro A, Llinares P, Canle D . Hospital outbreak caused by a carbapenem-resistant strain of Acinetobacter baumannii: patient prognosis and risk-factors for colonisation and infection. Clin Microbiol Infect. 2005; 11(7):540-6. DOI: 10.1111/j.1469-0691.2005.01184.x. View

Gales A, Jones R, Forward K, Linares J, Sader H, Verhoef J . Emerging importance of multidrug-resistant Acinetobacter species and Stenotrophomonas maltophilia as pathogens in seriously ill patients: geographic patterns, epidemiological features, and trends in the SENTRY Antimicrobial Surveillance Program.... Clin Infect Dis. 2001; 32 Suppl 2:S104-13. DOI: 10.1086/320183. View

Tysall L, Stockdale M, Chadwick P, Palepou M, Towner K, Livermore D . IMP-1 carbapenemase detected in an Acinetobacter clinical isolate from the UK. J Antimicrob Chemother. 2001; 49(1):217-8. DOI: 10.1093/jac/49.1.217. View

Walsh T, Bolmstrom A, Qwarnstrom A, Gales A . Evaluation of a new Etest for detecting metallo-beta-lactamases in routine clinical testing. J Clin Microbiol. 2002; 40(8):2755-9. PMC: 120685. DOI: 10.1128/JCM.40.8.2755-2759.2002. View

Zarrilli R, Crispino M, Bagattini M, Barretta E, Di Popolo A, Triassi M . Molecular epidemiology of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance. J Clin Microbiol. 2004; 42(3):946-53. PMC: 356829. DOI: 10.1128/JCM.42.3.946-953.2004. View

Doroshenko V, Livshits V . Structure and mode of transposition of Tn2555 carrying sucrose utilization genes. FEMS Microbiol Lett. 2004; 233(2):353-9. DOI: 10.1016/j.femsle.2004.03.016. View

Poirel L, Guibert M, Girlich D, Naas T, Nordmann P . Cloning, sequence analyses, expression, and distribution of ampC-ampR from Morganella morganii clinical isolates. Antimicrob Agents Chemother. 1999; 43(4):769-76. PMC: 89205. DOI: 10.1128/AAC.43.4.769. View

Poirel L, Marque S, Heritier C, Segonds C, Chabanon G, Nordmann P . OXA-58, a novel class D {beta}-lactamase involved in resistance to carbapenems in Acinetobacter baumannii. Antimicrob Agents Chemother. 2004; 49(1):202-8. PMC: 538857. DOI: 10.1128/AAC.49.1.202-208.2005. View

10.

Hammond D, Schooneveldt J, Nimmo G, Huygens F, Giffard P . bla(SHV) Genes in Klebsiella pneumoniae: different allele distributions are associated with different promoters within individual isolates. Antimicrob Agents Chemother. 2004; 49(1):256-63. PMC: 538876. DOI: 10.1128/AAC.49.1.256-263.2005. View

11.

Heritier C, Dubouix A, Poirel L, Marty N, Nordmann P . A nosocomial outbreak of Acinetobacter baumannii isolates expressing the carbapenem-hydrolysing oxacillinase OXA-58. J Antimicrob Chemother. 2004; 55(1):115-8. DOI: 10.1093/jac/dkh500. View

12.

Walsh T, Toleman M, Poirel L, Nordmann P . Metallo-beta-lactamases: the quiet before the storm?. Clin Microbiol Rev. 2005; 18(2):306-25. PMC: 1082798. DOI: 10.1128/CMR.18.2.306-325.2005. View

13.

Navon-Venezia S, Ben-Ami R, Carmeli Y . Update on Pseudomonas aeruginosa and Acinetobacter baumannii infections in the healthcare setting. Curr Opin Infect Dis. 2005; 18(4):306-13. DOI: 10.1097/01.qco.0000171920.44809.f0. View

14.

Heritier C, Poirel L, Lambert T, Nordmann P . Contribution of acquired carbapenem-hydrolyzing oxacillinases to carbapenem resistance in Acinetobacter baumannii. Antimicrob Agents Chemother. 2005; 49(8):3198-202. PMC: 1196226. DOI: 10.1128/AAC.49.8.3198-3202.2005. View

15.

Thomson J, Bonomo R . The threat of antibiotic resistance in Gram-negative pathogenic bacteria: beta-lactams in peril!. Curr Opin Microbiol. 2005; 8(5):518-24. DOI: 10.1016/j.mib.2005.08.014. View

16.

Hanlon G . The emergence of multidrug resistant Acinetobacter species: a major concern in the hospital setting. Lett Appl Microbiol. 2005; 41(5):375-8. DOI: 10.1111/j.1472-765X.2005.01791.x. View

17.

Murray C, Hospenthal D . Treatment of multidrug resistant Acinetobacter. Curr Opin Infect Dis. 2005; 18(6):502-6. DOI: 10.1097/01.qco.0000185985.64759.41. View

18.

Coelho J, Woodford N, Afzal-Shah M, Livermore D . Occurrence of OXA-58-like carbapenemases in Acinetobacter spp. collected over 10 years in three continents. Antimicrob Agents Chemother. 2006; 50(2):756-8. PMC: 1366923. DOI: 10.1128/AAC.50.2.756-758.2006. View

19.

Heritier C, Poirel L, Nordmann P . Cephalosporinase over-expression resulting from insertion of ISAba1 in Acinetobacter baumannii. Clin Microbiol Infect. 2006; 12(2):123-30. DOI: 10.1111/j.1469-0691.2005.01320.x. View

20.

Pournaras S, Markogiannakis A, Ikonomidis A, Kondyli L, Bethimouti K, Maniatis A . Outbreak of multiple clones of imipenem-resistant Acinetobacter baumannii isolates expressing OXA-58 carbapenemase in an intensive care unit. J Antimicrob Chemother. 2006; 57(3):557-61. DOI: 10.1093/jac/dkl004. View