Voltage-sensitive Prestin Orthologue Expressed in Zebrafish Hair Cells

Overview

Journal J Physiol

Specialty Physiology

Date 2007 Feb 3

PMID 17272340

Citations 32

Authors

Jorg T Albert

Harald Winter

Thorsten J Schaechinger

Thomas Weber

Xiang Wang

David Z Z He

Oliver Hendrich

Hyun-Soon Geisler

Ulrike Zimmermann

Katrin Oelmann

Marlies Knipper

Martin C Gopfert

Dominik Oliver

Affiliations

Soon will be listed here.

Abstract

Prestin, a member of the solute carrier (SLC) family SLC26A, is the molecular motor that drives the somatic electromotility of mammalian outer hair cells (OHCs). Its closest reported homologue, zebrafish prestin (zprestin), shares approximately 70% strong amino acid sequence similarity with mammalian prestin, predicting an almost identical protein structure. Immunohistochemical analysis now shows that zprestin is expressed in hair cells of the zebrafish ear. Similar to mammalian prestin, heterologously expressed zprestin is found to generate voltage-dependent charge movements, giving rise to a non-linear capacitance (NLC) of the cell membrane. Compared with mammalian prestin, charge movements mediated by zprestin display a weaker voltage dependence and slower kinetics; they occur at more positive membrane voltages, and are not associated with electromotile responses. Given this functional dissociation of NLC and electromotility and the structural similarity with mammalian prestin, we anticipate that zprestin provides a valuable tool for tracing the molecular and evolutionary bases of prestin motor function.

Citing Articles

Molecular specializations underlying phenotypic differences in inner ear hair cells of zebrafish and mice.

Giffen K, Liu H, Yamane K, Li Y, Chen L, Kramer K Front Neurol. 2024; 15:1437558.

PMID: 39484049 PMC: 11524865. DOI: 10.3389/fneur.2024.1437558.

Molecular Specializations Underlying Phenotypic Differences in Inner Ear Hair Cells of Zebrafish and Mice.

Giffen K, Liu H, Yamane K, Li Y, Chen L, Kramer K bioRxiv. 2024; .

PMID: 38826418 PMC: 11142236. DOI: 10.1101/2024.05.24.595729.

SLC26 Anion Transporters.

Geertsma E, Oliver D Handb Exp Pharmacol. 2023; 283:319-360.

PMID: 37947907 DOI: 10.1007/164_2023_698.

Elevator-like movements of prestin mediate outer hair cell electromotility.

Kuwabara M, Haddad B, Lenz-Schwab D, Hartmann J, Longo P, Huckschlag B Nat Commun. 2023; 14(1):7145.

PMID: 37932294 PMC: 10628124. DOI: 10.1038/s41467-023-42489-8.

State dependent effects on the frequency response of prestin's real and imaginary components of nonlinear capacitance.

Santos-Sacchi J, Navaratnam D, Tan W Sci Rep. 2021; 11(1):16149.

PMID: 34373481 PMC: 8352928. DOI: 10.1038/s41598-021-95121-4.

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