The Extracellular Loop of Pendrin and Prestin Modulates Their Voltage-sensing Property

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 2018 May 20

PMID 29777056

Citations 8

Authors

Makoto F Kuwabara

Koichiro Wasano

Satoe Takahashi

Justin Bodner

Tomotaka Komori

Sotaro Uemura

Jing Zheng

Tomohiro Shima

Kazuaki Homma

Affiliations

Soon will be listed here.

Abstract

Pendrin and prestin belong to the solute carrier 26 (SLC26) family of anion transporters. Prestin is unique among the SLC26 family members in that it displays voltage-driven motor activity (electromotility) and concurrent gating currents that manifest as nonlinear cell membrane electrical capacitance (nonlinear capacitance (NLC)). Although the anion transport mechanism of the SLC26 proteins has begun to be elucidated, the molecular mechanism of electromotility, which is thought to have evolved from an ancestral ion transport mechanism, still remains largely elusive. Here, we demonstrate that pendrin also exhibits large NLC and that charged residues present in one of the extracellular loops of pendrin and prestin play significant roles in setting the voltage-operating points of NLC. Our results suggest that the molecular mechanism responsible for sensing voltage is not unique to prestin among the members of the SLC26 family and that this voltage-sensing mechanism works independently of the anion transport mechanism.

Citing Articles

Cryo-EM structures of thermostabilized prestin provide mechanistic insights underlying outer hair cell electromotility.

Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S Nat Commun. 2022; 13(1):6208.

PMID: 36266333 PMC: 9584906. DOI: 10.1038/s41467-022-34017-x.

Single particle cryo-EM structure of the outer hair cell motor protein prestin.

Butan C, Song Q, Bai J, Tan W, Navaratnam D, Santos-Sacchi J Nat Commun. 2022; 13(1):290.

PMID: 35022426 PMC: 8755724. DOI: 10.1038/s41467-021-27915-z.

Identification and characterization of amphibian SLC26A5 using RNA-Seq.

Wang Z, Wang Q, Wu H, Huang Z BMC Genomics. 2021; 22(1):564.

PMID: 34294052 PMC: 8296623. DOI: 10.1186/s12864-021-07798-6.

An In Vitro Study on Prestin Analog Gene in the Bullfrog Hearing Organs.

Wang Z, Qian M, Wang Q, Liu H, Wu H, Huang Z Neural Plast. 2020; 2020:3570732.

PMID: 32714383 PMC: 7352134. DOI: 10.1155/2020/3570732.

Three-Dimensional Otic Neuronal Progenitor Spheroids Derived from Human Embryonic Stem Cells.

Heuer R, Nella K, Chang H, Coots K, Oleksijew A, Roque C Tissue Eng Part A. 2020; 27(3-4):256-269.

PMID: 32580647 PMC: 8045959. DOI: 10.1089/ten.TEA.2020.0078.

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