PRINS and C-PRINS: Promising Tools for the Physical Mapping of the Lupin Genome

Overview

Journal Cell Mol Biol Lett

Publisher Biomed Central

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2006 Nov 15

PMID 17103086

Citations 4

Authors

Anna Kaczmarek

Barbara Naganowska

Bogdan Wolko

Affiliations

Soon will be listed here.

Abstract

Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification in lupin.

Citing Articles

Comparative genomics of Lupinus angustifolius gene-rich regions: BAC library exploration, genetic mapping and cytogenetics.

Ksiazkiewicz M, Wyrwa K, Szczepaniak A, Rychel S, Majcherkiewicz K, Przysiecka L BMC Genomics. 2013; 14:79.

PMID: 23379841 PMC: 3618312. DOI: 10.1186/1471-2164-14-79.

Development of genomic resources for the narrow-leafed lupin (Lupinus angustifolius): construction of a bacterial artificial chromosome (BAC) library and BAC-end sequencing.

Gao L, Hane J, Kamphuis L, Foley R, Shi B, Atkins C BMC Genomics. 2011; 12:521.

PMID: 22014081 PMC: 3206524. DOI: 10.1186/1471-2164-12-521.

Identification and functional analysis of PCNA1 and PCNA-like1 genes of Phaseolus coccineus.

Strzalka W, Kaczmarek A, Naganowska B, Ziemienowicz A J Exp Bot. 2009; 61(3):873-88.

PMID: 20007687 PMC: 2814116. DOI: 10.1093/jxb/erp354.

Karyotyping of the narrow-leafed lupin (Lupinus angustifolius L.) by using FISH, PRINS and computer measurements of chromosomes.

Kaczmarek A, Naganowska B, Wolko B J Appl Genet. 2009; 50(2):77-82.

PMID: 19433903 DOI: 10.1007/BF03195657.

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