Development of Genomic Resources for the Narrow-leafed Lupin (Lupinus Angustifolius): Construction of a Bacterial Artificial Chromosome (BAC) Library and BAC-end Sequencing

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2011 Oct 22

PMID 22014081

Citations 29

Authors

Ling-Ling Gao

James K Hane

Lars G Kamphuis

Rhonda Foley

Bu-Jun Shi

Craig A Atkins

Karam B Singh

Affiliations

Soon will be listed here.

Abstract

Background: Lupinus angustifolius L, also known as narrow-leafed lupin (NLL), is becoming an important grain legume crop that is valuable for sustainable farming and is becoming recognised as a potential human health food. Recent interest is being directed at NLL to improve grain production, disease and pest management and health benefits of the grain. However, studies have been hindered by a lack of extensive genomic resources for the species.

Results: A NLL BAC library was constructed consisting of 111,360 clones with an average insert size of 99.7 Kbp from cv Tanjil. The library has approximately 12 × genome coverage. Both ends of 9600 randomly selected BAC clones were sequenced to generate 13985 BAC end-sequences (BESs), covering approximately 1% of the NLL genome. These BESs permitted a preliminary characterisation of the NLL genome such as organisation and composition, with the BESs having approximately 39% G:C content, 16.6% repetitive DNA and 5.4% putative gene-encoding regions. From the BESs 9966 simple sequence repeat (SSR) motifs were identified and some of these are shown to be potential markers.

Conclusions: The NLL BAC library and BAC-end sequences are powerful resources for genetic and genomic research on lupin. These resources will provide a robust platform for future high-resolution mapping, map-based cloning, comparative genomics and assembly of whole-genome sequencing data for the species.

Citing Articles

A pan-genome and chromosome-length reference genome of narrow-leafed lupin (Lupinus angustifolius) reveals genomic diversity and insights into key industry and biological traits.

Garg G, Kamphuis L, Bayer P, Kaur P, Dudchenko O, Taylor C Plant J. 2022; 111(5):1252-1266.

PMID: 35779281 PMC: 9544533. DOI: 10.1111/tpj.15885.

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin ( L.).

Rychel-Bielska S, Plewinski P, Kozak B, Galek R, Ksia Zkiewicz M Front Plant Sci. 2020; 11:572135.

PMID: 33193508 PMC: 7663182. DOI: 10.3389/fpls.2020.572135.

A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin ( L.).

Czyz K, Ksiazkiewicz M, Koczyk G, Szczepaniak A, Podkowinski J, Naganowska B Int J Mol Sci. 2020; 21(7).

PMID: 32276381 PMC: 7177731. DOI: 10.3390/ijms21072580.

Candidate Domestication-Related Genes Revealed by Expression Quantitative Trait Loci Mapping of Narrow-Leafed Lupin ( L.).

Plewinski P, Ksiazkiewicz M, Rychel-Bielska S, Rudy E, Wolko B Int J Mol Sci. 2019; 20(22).

PMID: 31726789 PMC: 6888189. DOI: 10.3390/ijms20225670.

Validation of Diaporthe toxica resistance markers in European Lupinus angustifolius germplasm and identification of novel resistance donors for marker-assisted selection.

Ksiazkiewicz M, Wojcik K, Irzykowski W, Bielski W, Rychel S, Kaczmarek J J Appl Genet. 2019; 61(1):1-12.

PMID: 31641945 PMC: 6968985. DOI: 10.1007/s13353-019-00521-y.

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