The Bacterial Artificial Chromosome (BAC) Library of the Narrow-leafed Lupin (Lupinus Angustifolius L.)

Overview

Journal Cell Mol Biol Lett

Publisher Biomed Central

Specialties Biochemistry
Cell Biology
Molecular Biology

Date 2006 Jul 19

PMID 16847554

Citations 26

Authors

Andrzej Kasprzak

Jan Safar

Jaroslav Janda

Jaroslav Dolezel

Bogdan Wolko

Barbara Naganowska

Affiliations

Soon will be listed here.

Abstract

The narrow-leafed lupin possesses valuable traits for environment-friendly agriculture and for the production of unconventional agricultural products. Despite various genetic and environmental studies, the breeding of improved cultivars has been slow due to the limited knowledge of its genomic structure. Further advances in genomics require, among other things, the availability of a genomic DNA library with large inserts. We report here on the construction of the first DNA library cloned in a BAC (bacterial artificial chromosome) vector from diploid Lupinus angustifolius L. cv. Sonet. The high molecular weight DNA used for its preparation was isolated from interphase nuclei that were purified by flow cytometry. The library comprises 55,296 clones and is ordered in 144x384-well microtitre plates. With an average insert size of 100 kb, the library represents six haploid genome equivalents. Thanks to the purification of the nuclei by flow cytometry, contamination with chloroplast DNA and mitochondrial DNA was negligible. The availability of a BAC library opens avenues for the development of a physical contig map and positional gene cloning, as well as for the analysis of the plant's genome structure and evolution.

Citing Articles

A pan-genome and chromosome-length reference genome of narrow-leafed lupin (Lupinus angustifolius) reveals genomic diversity and insights into key industry and biological traits.

Garg G, Kamphuis L, Bayer P, Kaur P, Dudchenko O, Taylor C Plant J. 2022; 111(5):1252-1266.

PMID: 35779281 PMC: 9544533. DOI: 10.1111/tpj.15885.

Progress of Genomics-Driven Approaches for Sustaining Underutilized Legume Crops in the Post-Genomic Era.

Jha U, Nayyar H, Parida S, Bakir M, von Wettberg E, Siddique K Front Genet. 2022; 13:831656.

PMID: 35464848 PMC: 9021634. DOI: 10.3389/fgene.2022.831656.

The Puzzling Fate of a Lupin Chromosome Revealed by Reciprocal Oligo-FISH and BAC-FISH Mapping.

Bielski W, Ksiazkiewicz M, Simonikova D, Hribova E, Susek K, Naganowska B Genes (Basel). 2020; 11(12).

PMID: 33322080 PMC: 7764521. DOI: 10.3390/genes11121489.

Photoperiod and Vernalization Control of Flowering-Related Genes: A Case Study of the Narrow-Leafed Lupin ( L.).

Rychel-Bielska S, Plewinski P, Kozak B, Galek R, Ksia Zkiewicz M Front Plant Sci. 2020; 11:572135.

PMID: 33193508 PMC: 7663182. DOI: 10.3389/fpls.2020.572135.

A Tale of Two Families: Whole Genome and Segmental Duplications Underlie Glutamine Synthetase and Phosphoenolpyruvate Carboxylase Diversity in Narrow-Leafed Lupin ( L.).

Czyz K, Ksiazkiewicz M, Koczyk G, Szczepaniak A, Podkowinski J, Naganowska B Int J Mol Sci. 2020; 21(7).

PMID: 32276381 PMC: 7177731. DOI: 10.3390/ijms21072580.

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