Identification and Functional Analysis of PCNA1 and PCNA-like1 Genes of Phaseolus Coccineus

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2009 Dec 17

PMID 20007687

Citations 8

Authors

Wojciech Strzalka

Anna Kaczmarek

Barbara Naganowska

Alicja Ziemienowicz

Affiliations

Soon will be listed here.

Abstract

Proliferating cell nuclear antigen (PCNA) is an essential factor in DNA replication and in many other processes in eukaryotic cells. Genetic analysis of Phaseolus coccineus showed the presence of at least two PCNA-like genes in the runner bean genome. Two PCNA genes have previously been found in a few plant species including Arabidopsis, tobacco, and maize. In these species, genes were nearly identical. Two cDNAs of P. coccineus PCNA (PcPCNA1 and PcPCNA-like1) have been identified that differ distinctly from each other. Interestingly, both the genetic organization of PcPCNA1 and PcPCNA-like1 genes and their expression patterns were similar, but these were the only similarities between these genes and their products. The identity between PcPCNA1 and PcPCNA-like1 at the amino acid level was only 54%, with PcPCNA-like1 lacking motifs that are crucial for the activity typical of PCNA. Consequently, these two proteins showed different properties. PcPCNA1 behaved like a typical PCNA protein: it formed a homotrimer and stimulated the activity of human DNA polymerase delta. In addition, PcPCNA1 interacted with a p21 peptide and was recognized by an anti-human PCNA monoclonal antibody PC10. By contrast, PcPCNA-like1 was detected as a monomer and was unable to stimulate the DNA polymerase delta activity. PcPCNA-like1 also could not interact with p21 and was not recognized by the PC10 antibody. Our results suggest that PcPCNA-like1 either is unable to function alone and therefore might be a component of the heterotrimeric PCNA ring or may have other, yet unknown functions. Alternatively, the PcPCNA-like1 gene may represent a pseudogene.

Citing Articles

The Dark Side of UV-Induced DNA Lesion Repair.

Strzalka W, Zglobicki P, Kowalska E, Bazant A, Dziga D, Banas A Genes (Basel). 2020; 11(12).

PMID: 33276692 PMC: 7761550. DOI: 10.3390/genes11121450.

Toxic effect and mechanism of four ionic liquids on seedling taproots of Arabidopsis thaliana.

Li M, Xue Y, Liu Z, Guo J, Liu L, Zhang Y Environ Sci Pollut Res Int. 2018; 25(15):14703-14712.

PMID: 29532385 DOI: 10.1007/s11356-018-1621-2.

Thioredoxin (Trxo1) interacts with proliferating cell nuclear antigen (PCNA) and its overexpression affects the growth of tobacco cell culture.

Calderon A, Ortiz-Espin A, Iglesias-Fernandez R, Carbonero P, Pallardo F, Sevilla F Redox Biol. 2017; 11:688-700.

PMID: 28183062 PMC: 5299145. DOI: 10.1016/j.redox.2017.01.018.

Arabidopsis PCNAs form complexes with selected D-type cyclins.

Strzalka W, Aggarwal C, Krzeszowiec W, Jakubowska A, Sztatelman O, Banas A Front Plant Sci. 2015; 6:516.

PMID: 26379676 PMC: 4550699. DOI: 10.3389/fpls.2015.00516.

Arabidopsis thaliana: proliferating cell nuclear antigen 1 and 2 possibly form homo- and hetero-trimeric complexes in the plant cell.

Strzalka W, Aggarwal C Plant Signal Behav. 2013; 8(7):e24837.

PMID: 23656863 PMC: 3909058. DOI: 10.4161/psb.24837.

References

Weiser T, Gassmann M, Thommes P, Ferrari E, Hafkemeyer P, Hubscher U . Biochemical and functional comparison of DNA polymerases alpha, delta, and epsilon from calf thymus. J Biol Chem. 1991; 266(16):10420-8. View

Capel B, Swain A, Nicolis S, Hacker A, Walter M, Koopman P . Circular transcripts of the testis-determining gene Sry in adult mouse testis. Cell. 1993; 73(5):1019-30. DOI: 10.1016/0092-8674(93)90279-y. View

Strzalka W, Oyama T, Tori K, Morikawa K . Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment. Protein Sci. 2009; 18(5):1072-80. PMC: 2771309. DOI: 10.1002/pro.117. View

Laquel P, Litvak S, Castroviejo M . Mammalian proliferating cell nuclear antigen stimulates the processivity of two wheat embryo DNA polymerases. Plant Physiol. 1993; 102(1):107-14. PMC: 158752. DOI: 10.1104/pp.102.1.107. View

Hirotsune S, Yoshida N, Chen A, Garrett L, Sugiyama F, Takahashi S . An expressed pseudogene regulates the messenger-RNA stability of its homologous coding gene. Nature. 2003; 423(6935):91-6. DOI: 10.1038/nature01535. View

Koch J, Kolvraa S, Petersen K, Gregersen N, Bolund L . Oligonucleotide-priming methods for the chromosome-specific labelling of alpha satellite DNA in situ. Chromosoma. 1989; 98(4):259-65. DOI: 10.1007/BF00327311. View

Kosugi S, Ohashi Y . E2F sites that can interact with E2F proteins cloned from rice are required for meristematic tissue-specific expression of rice and tobacco proliferating cell nuclear antigen promoters. Plant J. 2002; 29(1):45-59. DOI: 10.1046/j.1365-313x.2002.01196.x. View

Guerini M, Behnke M, White M . Biochemical and genetic analysis of the distinct proliferating cell nuclear antigens of Toxoplasma gondii. Mol Biochem Parasitol. 2005; 142(1):56-65. DOI: 10.1016/j.molbiopara.2005.03.020. View

Maeda T, Chong M, Espino R, Chua P, Cao J, Chomey E . Role of p21(Waf-1) in regulating the G1 and G2/M checkpoints in ultraviolet-irradiated keratinocytes. J Invest Dermatol. 2002; 119(2):513-21. DOI: 10.1046/j.1523-1747.2002.01828.x. View

10.

Zhang P, Sun Y, Hsu H, Zhang L, Zhang Y, Lee M . The interdomain connector loop of human PCNA is involved in a direct interaction with human polymerase delta. J Biol Chem. 1998; 273(2):713-9. DOI: 10.1074/jbc.273.2.713. View

11.

Miyachi K, Fritzler M, Tan E . Autoantibody to a nuclear antigen in proliferating cells. J Immunol. 1978; 121(6):2228-34. View

12.

Pizzuti A, Novelli G, Ratti A, Amati F, Bordoni R, Mandich P . Isolation and characterization of a novel transcript embedded within HIRA, a gene deleted in DiGeorge syndrome. Mol Genet Metab. 1999; 67(3):227-35. DOI: 10.1006/mgme.1999.2868. View

13.

Markley N, Bonham-Smith P, Moloney M . Molecular cloning and expression of a cDNA encoding the proliferating cell nuclear antigen from Brassica napus (oilseed rape). Genome. 1993; 36(3):459-66. DOI: 10.1139/g93-063. View

14.

Strzalka W, Ziemienowicz A . Molecular cloning of Phaseolus vulgaris cDNA encoding proliferating cell nuclear antigen. J Plant Physiol. 2006; 164(2):209-13. DOI: 10.1016/j.jplph.2006.04.009. View

15.

Dolezel J, Cihalikova J, Weiserova J, Lucretti S . Cell cycle synchronization in plant root meristems. Methods Cell Sci. 2000; 21(2-3):95-107. DOI: 10.1023/a:1009876621187. View

16.

Leibovici M, Gusse M, Bravo R, Mechali M . Characterization and developmental expression of Xenopus proliferating cell nuclear antigen (PCNA). Dev Biol. 1990; 141(1):183-92. DOI: 10.1016/0012-1606(90)90113-w. View

17.

Kandouz M, Bier A, Carystinos G, Alaoui-Jamali M, Batist G . Connexin43 pseudogene is expressed in tumor cells and inhibits growth. Oncogene. 2004; 23(27):4763-70. DOI: 10.1038/sj.onc.1207506. View

18.

SUZUKA I, Hata S, Matsuoka M, Kosugi S, Hashimoto J . Highly conserved structure of proliferating cell nuclear antigen (DNA polymerase delta auxiliary protein) gene in plants. Eur J Biochem. 1991; 195(2):571-5. DOI: 10.1111/j.1432-1033.1991.tb15739.x. View

19.

Ball K, Lane D . Human and plant proliferating-cell nuclear antigen have a highly conserved binding site for the p53-inducible gene product p21WAF1. Eur J Biochem. 1996; 237(3):854-61. DOI: 10.1111/j.1432-1033.1996.0854p.x. View

20.

Kaczmarek A, Naganowska B, Wolko B . PRINS and C-PRINS: promising tools for the physical mapping of the lupin genome. Cell Mol Biol Lett. 2006; 12(1):16-24. PMC: 6275731. DOI: 10.2478/s11658-006-0056-9. View