Physical Map of the Chromosome of Lactococcus Lactis Subsp. Lactis DL11 and Localization of Six Putative RRNA Operons

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 May 1

PMID 1708377

Citations 18

Authors

D L Tulloch

L R FINCH

A J Hillier

B E Davidson

Affiliations

Soon will be listed here.

Abstract

A physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 was constructed by using the contour-clamped homogeneous electric field mode of pulsed-field gel electrophoresis in one- and two-dimensional separations to analyze restriction digests of high-molecular-weight genomic DNA. The map, which shows all the observed NotI and SmaI sites (six and 21, respectively) and 8 of approximately 30 SalI sites, is circular and yields a total size of 2.58 megabase pairs for the L. lactis subsp. lactis DL11 chromosome. By using rDNA from Mycoplasma capricolum to probe Southern blots of pulsed-and fixed-field digestion patterns, six putative rRNA operons were identified in L. lactis subsp. lactis DL11 and placed on the map of the chromosome. Five of these loci are clustered in a region representing only 20% of the chromosome. The presence of a SmaI site in each of the putative operons allowed the direction of transcription of each operon to be deduced.

Citing Articles

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Construction and initial characterization of Escherichia coli strains with few or no intact chromosomal rRNA operons.

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A natural large chromosomal inversion in Lactococcus lactis is mediated by homologous recombination between two insertion sequences.

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References

Terzaghi B, Sandine W . Improved medium for lactic streptococci and their bacteriophages. Appl Microbiol. 1975; 29(6):807-13. PMC: 187084. DOI: 10.1128/am.29.6.807-813.1975. View

Kiss A, Sain B, VENETIANER P . The number of rRNA genes in Escherichia coli. FEBS Lett. 1977; 79(1):77-9. DOI: 10.1016/0014-5793(77)80354-2. View

McKay L, Bohanon M, Polzin K, Rule P, Baldwin K . Localization of Separate Genetic Loci for Reduced Sensitivity towards Small Isometric-Headed Bacteriophage sk1 and Prolate-Headed Bacteriophage c2 on pGBK17 from Lactococcus lactis subsp. lactis KR2. Appl Environ Microbiol. 1989; 55(10):2702-9. PMC: 203147. DOI: 10.1128/aem.55.10.2702-2709.1989. View

Tanskanen E, Tulloch D, Hillier A, Davidson B . Pulsed-Field Gel Electrophoresis of SmaI Digests of Lactococcal Genomic DNA, a Novel Method of Strain Identification. Appl Environ Microbiol. 1990; 56(10):3105-11. PMC: 184906. DOI: 10.1128/aem.56.10.3105-3111.1990. View

Jarvis A, Jarvis B . Deoxyribonucleic Acid homology among lactic streptococci. Appl Environ Microbiol. 1981; 41(1):77-83. PMC: 243642. DOI: 10.1128/aem.41.1.77-83.1981. View

Kok J, Leenhouts K, Haandrikman A, Ledeboer A, Venema G . Nucleotide sequence of the cell wall proteinase gene of Streptococcus cremoris Wg2. Appl Environ Microbiol. 1988; 54(1):231-8. PMC: 202426. DOI: 10.1128/aem.54.1.231-238.1988. View

Saiki R, Gelfand D, Stoffel S, Scharf S, Higuchi R, Horn G . Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988; 239(4839):487-91. DOI: 10.1126/science.2448875. View

Ventra L, Weiss A . Transposon-mediated restriction mapping of the Bacillus subtilis chromosome. Gene. 1989; 78(1):29-36. DOI: 10.1016/0378-1119(89)90311-9. View

Brewer B . When polymerases collide: replication and the transcriptional organization of the E. coli chromosome. Cell. 1988; 53(5):679-86. DOI: 10.1016/0092-8674(88)90086-4. View

10.

Jarvis E, Widom R, LaFauci G, Setoguchi Y, Richter I, Rudner R . Chromosomal organization of rRNA operons in Bacillus subtilis. Genetics. 1988; 120(3):625-35. PMC: 1203540. DOI: 10.1093/genetics/120.3.625. View

11.

PYLE L, FINCH L . A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci. Nucleic Acids Res. 1988; 16(13):6027-39. PMC: 336845. DOI: 10.1093/nar/16.13.6027. View

12.

Hartmann R, Toschka H, Ulbrich N, Erdmann V . Genomic organization of rDNA in Pseudomonas aeruginosa. FEBS Lett. 1986; 195(1-2):187-93. DOI: 10.1016/0014-5793(86)80158-2. View

13.

Vollrath D, Davis R . Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res. 1987; 15(19):7865-76. PMC: 306313. DOI: 10.1093/nar/15.19.7865. View

14.

Boizet B, Villeval D, Slos P, NOVEL M, Novel G, Mercenier A . Isolation and structural analysis of the phospho-beta-galactosidase gene from Streptococcus lactis Z268. Gene. 1988; 62(2):249-61. DOI: 10.1016/0378-1119(88)90563-x. View

15.

Dams E, Hendriks L, Van de Peer Y, Neefs J, Smits G, Vandenbempt I . Compilation of small ribosomal subunit RNA sequences. Nucleic Acids Res. 1988; 16 Suppl:r87-173. PMC: 340911. DOI: 10.1093/nar/16.suppl.r87. View

16.

van der Vossen J, van der Lelie D, Venema G . Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol. 1987; 53(10):2452-7. PMC: 204128. DOI: 10.1128/aem.53.10.2452-2457.1987. View

17.

Canard B, Cole S . Genome organization of the anaerobic pathogen Clostridium perfringens. Proc Natl Acad Sci U S A. 1989; 86(17):6676-80. PMC: 297908. DOI: 10.1073/pnas.86.17.6676. View

18.

Ross R, OGara F, Condon S . Cloning of chromosomal genes of Lactococcus by heterologous complementation: partial characterisation of a putative lactose transport gene. FEMS Microbiol Lett. 1989; 52(1-2):183-7. DOI: 10.1016/0378-1097(89)90193-6. View

19.

Romling U, Grothues D, Bautsch W, Tummler B . A physical genome map of Pseudomonas aeruginosa PAO. EMBO J. 1989; 8(13):4081-9. PMC: 401585. DOI: 10.1002/j.1460-2075.1989.tb08592.x. View

20.

Kauc L, Mitchell M, GOODGAL S . Size and physical map of the chromosome of Haemophilus influenzae. J Bacteriol. 1989; 171(5):2474-9. PMC: 209923. DOI: 10.1128/jb.171.5.2474-2479.1989. View