Physical and Genetic Map of the Clostridium Acetobutylicum ATCC 824 Chromosome

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1997 Dec 11

PMID 9393708

Citations 7

Authors

E Cornillot

C Croux

P Soucaille

Affiliations

Soon will be listed here.

Abstract

A physical and genetic map of the Clostridium acetobutylicum ATCC 824 chromosome was constructed. The macrorestriction map for CeuI, EagI, and SstII was created by ordering the 38 restriction sites by one- and two-dimensional pulsed-field gel electrophoresis (PFGE) and by using an original strategy based on the CeuI enzyme and indirect end labelling by hybridization on both sides of the CeuI sites with rrs (16S RNA) and 3' rrl (23S RNA) probes. The circular chromosome was estimated to be 4.15 Mb in size, and the average resolution of the physical map is 110 kb. The chromosome contains 11 rrn loci, which are localized on 44% of the chromosome in a divergent transcriptional orientation regarding the presumed location of the replication origin. In addition to these 11 rrn operons, a total of 40 identified genes were mapped by hybridization experiments with genes from C. acetobutylicum and from various other clostridia as probes. The genetic map of C. acetobutylicum was compared to that of the three other endospore-forming bacteria characterized so far: Bacillus subtilis, Clostridium beijerinckii, and Clostridium perfringens. Parodoxically, the chromosomal backbone of C. acetobutylicum showed more similarity to that of B. subtilis than to those of the clostridia.

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References

Gerischer U, Durre P . Cloning, sequencing, and molecular analysis of the acetoacetate decarboxylase gene region from Clostridium acetobutylicum. J Bacteriol. 1990; 172(12):6907-18. PMC: 210810. DOI: 10.1128/jb.172.12.6907-6918.1990. View

Walter K, Bennett G, Papoutsakis E . Molecular characterization of two Clostridium acetobutylicum ATCC 824 butanol dehydrogenase isozyme genes. J Bacteriol. 1992; 174(22):7149-58. PMC: 207405. DOI: 10.1128/jb.174.22.7149-7158.1992. View

Schwartz D, Cantor C . Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984; 37(1):67-75. DOI: 10.1016/0092-8674(84)90301-5. View

Cornillot E, Daube G, Katayama S, Granum P, Canard B, Cole S . The enterotoxin gene (cpe) of Clostridium perfringens can be chromosomal or plasmid-borne. Mol Microbiol. 1995; 15(4):639-47. DOI: 10.1111/j.1365-2958.1995.tb02373.x. View

Cornillot E, Nair R, Papoutsakis E, Soucaille P . The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. J Bacteriol. 1997; 179(17):5442-7. PMC: 179415. DOI: 10.1128/jb.179.17.5442-5447.1997. View

Schmidt K, Tummler B, Romling U . Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats. J Bacteriol. 1996; 178(1):85-93. PMC: 177624. DOI: 10.1128/jb.178.1.85-93.1996. View

Boynton Z, Bennett G, Rudolph F . Cloning, sequencing, and expression of genes encoding phosphotransacetylase and acetate kinase from Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol. 1996; 62(8):2758-66. PMC: 168061. DOI: 10.1128/aem.62.8.2758-2766.1996. View

Petersen D, Bennett G . Characterization of an acetyl-CoA C-acetyltransferase (thiolase) gene from Clostridium acetobutylicum ATCC 824. Gene. 1995; 154(1):81-5. DOI: 10.1016/0378-1119(94)00838-j. View

Fischer R, Helms J, Durre P . Cloning, sequencing, and molecular analysis of the sol operon of Clostridium acetobutylicum, a chromosomal locus involved in solventogenesis. J Bacteriol. 1993; 175(21):6959-69. PMC: 206823. DOI: 10.1128/jb.175.21.6959-6969.1993. View

10.

Gottlieb P, LaFauci G, Rudner R . Alterations in the number of rRNA operons within the Bacillus subtilis genome. Gene. 1985; 33(3):259-68. DOI: 10.1016/0378-1119(85)90233-1. View

11.

Cole S, Saint Girons I . Bacterial genomics. FEMS Microbiol Rev. 1994; 14(2):139-60. DOI: 10.1111/j.1574-6976.1994.tb00084.x. View

12.

Salazar L, Fsihi H, De Rossi E, Riccardi G, Rios C, Cole S . Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis. Mol Microbiol. 1996; 20(2):283-93. DOI: 10.1111/j.1365-2958.1996.tb02617.x. View

13.

Katayama S, Dupuy B, Garnier T, Cole S . Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50. J Bacteriol. 1995; 177(19):5680-5. PMC: 177380. DOI: 10.1128/jb.177.19.5680-5685.1995. View

14.

Garnier T, Canard B, Cole S . Cloning, mapping, and molecular characterization of the rRNA operons of Clostridium perfringens. J Bacteriol. 1991; 173(17):5431-8. PMC: 208255. DOI: 10.1128/jb.173.17.5431-5438.1991. View

15.

Narberhaus F, Giebeler K, Bahl H . Molecular characterization of the dnaK gene region of Clostridium acetobutylicum, including grpE, dnaJ, and a new heat shock gene. J Bacteriol. 1992; 174(10):3290-9. PMC: 205998. DOI: 10.1128/jb.174.10.3290-3299.1992. View

16.

Boynton Z, Bennet G, Rudolph F . Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. J Bacteriol. 1996; 178(11):3015-24. PMC: 178046. DOI: 10.1128/jb.178.11.3015-3024.1996. View

17.

Tulloch D, FINCH L, Hillier A, Davidson B . Physical map of the chromosome of Lactococcus lactis subsp. lactis DL11 and localization of six putative rRNA operons. J Bacteriol. 1991; 173(9):2768-75. PMC: 207856. DOI: 10.1128/jb.173.9.2768-2775.1991. View

18.

Meyer J . Cloning and sequencing of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum. Biochim Biophys Acta. 1993; 1174(1):108-10. DOI: 10.1016/0167-4781(93)90102-j. View

19.

Keis S, Bennett C, Ward V, Jones D . Taxonomy and phylogeny of industrial solvent-producing clostridia. Int J Syst Bacteriol. 1995; 45(4):693-705. DOI: 10.1099/00207713-45-4-693. View

20.

Roussel Y, Pebay M, Guedon G, Simonet J, Decaris B . Physical and genetic map of Streptococcus thermophilus A054. J Bacteriol. 1994; 176(24):7413-22. PMC: 197195. DOI: 10.1128/jb.176.24.7413-7422.1994. View