Size and Physical Map of the Chromosome of Haemophilus Influenzae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1989 May 1

PMID 2785105

Citations 29

Authors

L Kauc

M Mitchell

S H GOODGAL

Affiliations

Soon will be listed here.

Abstract

A variation of pulse-field electrophoresis, field-inversion gel electrophoresis, was used to determine the size and physical map of the chromosome of Haemophilus influenzae. The DNA of H. influenzae had a low G + C content (39%) and no restriction sites for the enzymes NotI or SfiI. However, a number of restriction enzymes (SmaI, ApaI, NaeI, and SacII) that recognized 6-base-pair sequences containing only G and C nucleotides were found to generate a reasonable number of DNA fragments that were separable in agarose gels by field-inversion gel electrophoresis. The sizes of the DNA fragments were calibrated with a lambda DNA ladder and lambda DNA restriction fragments. The sum of fragment sizes obtained with restriction digests yielded a value for the chromosome of 1,980 kilobase pairs. Hybridization of a labeled fragment with two or more fragments from a digest with a different restriction enzyme provided the information needed to construct a circular map of the H. influenzae chromosome.

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References

Schwartz D, Cantor C . Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984; 37(1):67-75. DOI: 10.1016/0092-8674(84)90301-5. View

Zoon K, SCOCCA J . Constitution of the cell envelope of Haemophilus influenzae in relation to competence for genetic transformation. J Bacteriol. 1975; 123(2):666-77. PMC: 235774. DOI: 10.1128/jb.123.2.666-677.1975. View

Jackson D, Cook P . A general method for preparing chromatin containing intact DNA. EMBO J. 1985; 4(4):913-8. PMC: 554279. DOI: 10.1002/j.1460-2075.1985.tb03718.x. View

Waldman A, Fitzmaurice W, SCOCCA J . Integration of the bacteriophage HP1c1 genome into the Haemophilus influenzae Rd chromosome in the lysogenic state. J Bacteriol. 1986; 165(1):297-300. PMC: 214404. DOI: 10.1128/jb.165.1.297-300.1986. View

Carle G, Frank M, Olson M . Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science. 1986; 232(4746):65-8. DOI: 10.1126/science.3952500. View

Waterbury P, Lane M . Generation of lambda phage concatemers for use as pulsed field electrophoresis size markers. Nucleic Acids Res. 1987; 15(9):3930. PMC: 340804. DOI: 10.1093/nar/15.9.3930. View

Smith C, Econome J, Schutt A, Klco S, Cantor C . A physical map of the Escherichia coli K12 genome. Science. 1987; 236(4807):1448-53. DOI: 10.1126/science.3296194. View

Cantor C, Smith C, Mathew M . Pulsed-field gel electrophoresis of very large DNA molecules. Annu Rev Biophys Biophys Chem. 1988; 17:287-304. DOI: 10.1146/annurev.bb.17.060188.001443. View

Lee J, Smith H . Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. J Bacteriol. 1988; 170(9):4402-5. PMC: 211461. DOI: 10.1128/jb.170.9.4402-4405.1988. View

10.

Kauc L, GOODGAL S . Amplification of DNA at a prophage attachment site in Haemophilus influenzae. J Bacteriol. 1989; 171(4):1898-903. PMC: 209838. DOI: 10.1128/jb.171.4.1898-1903.1989. View

11.

Alexander H, Leidy G . Determination of inherited traits of H. influenzae by desoxyribonucleic acid fractions isolated from type-specific cells. J Exp Med. 1951; 93(4):345-59. PMC: 2136084. DOI: 10.1084/jem.93.4.345. View

12.

ZAMENHOF S, Alexander H, Leidy G . Studies on the chemistry of the transforming activity. I. Resistance to physical and chemical agents. J Exp Med. 1953; 98(4):373-97. PMC: 2136247. DOI: 10.1084/jem.98.4.373. View

13.

Berns K, Thomas Jr C . ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965; 11:476-90. DOI: 10.1016/s0022-2836(65)80004-3. View

14.

Michalka J, GOODGAL S . Genetic and physical map of the chromosome of Hemophilus influenzae. J Mol Biol. 1969; 45(2):407-21. DOI: 10.1016/0022-2836(69)90115-6. View

15.

STUY J . Transfer of genetic information within a colony of Haemophilus influenzae. J Bacteriol. 1985; 162(1):1-4. PMC: 218943. DOI: 10.1128/jb.162.1.1-4.1985. View