Formation of GW Bodies is a Consequence of MicroRNA Genesis

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2006 Aug 15

PMID 16906129

Citations 53

Authors

Kaleb M Pauley

Theophany Eystathioy

Andrew Jakymiw

John C Hamel

Marvin J Fritzler

Edward K L Chan

Affiliations

Soon will be listed here.

Abstract

GW bodies (GWBs), or mammalian P bodies, proposed to be involved in messenger RNA storage and/or degradation, have recently been linked to RNA interference and microRNA (miRNA) processing. We report that endogenous let-7 miRNA co-precipitates with the GW182 protein complex. In addition, knockdown of two proteins, Drosha and its protein partner DGCR8, which are vital to the generation of mature miRNA, results in the loss of GWBs. Subsequent introduction of short interference RNA specific to lamin A/C is accompanied by reassembly of GWBs and concurrent knockdown of lamin A/C protein. Taken together, these studies show that miRNAs are crucial components in GWB formation.

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