The GW182 Protein Colocalizes with MRNA Degradation Associated Proteins HDcp1 and HLSm4 in Cytoplasmic GW Bodies

Overview

Journal RNA

Specialty Molecular Biology

Date 2003 Sep 18

PMID 13130130

Citations 139

Authors

Theophany Eystathioy

Andrew Jakymiw

Edward K L Chan

Bertrand Seraphin

Nicolas Cougot

Marvin J Fritzler

Affiliations

Soon will be listed here.

Abstract

A novel cytoplasmic compartment referred to as GW bodies (GWBs) was initially identified using antibodies specific to a 182-kD protein termed GW182. GW182 was characterized by multiple glycine(G)-tryptophan(W) repeats and an RNA recognition motif (RRM) that bound a subset of HeLa cell messenger RNAs (mRNAs). The function of GWBs was not known; however, more recent evidence suggested similarities between GWBs and cytoplasmic structures that contain hLSm proteins and hDcp1, the human homolog to a yeast decapping enzyme subunit. In this study, we used antibodies to hLSm4 and hDcp1 to show that both of these markers of an mRNA degradation pathway colocalize to the same structures as GW182. Our studies demonstrate that GW182, hLSm4, and hDcp1 are found in the same cytoplasmic structures and suggest that GW182 is involved in the same mRNA processing pathway as hLSm4 and hDcp1.

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