A Candidate Approach Implicates the Secreted Salmonella Effector Protein SpvB in P-body Disassembly

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2011 Mar 11

PMID 21390246

Citations 15

Authors

Ana Eulalio

Kathrin S Frohlich

Miguel Mano

Mauro Giacca

Jorg Vogel

Affiliations

Soon will be listed here.

Abstract

P-bodies are dynamic aggregates of RNA and proteins involved in several post-transcriptional regulation processes. P-bodies have been shown to play important roles in regulating viral infection, whereas their interplay with bacterial pathogens, specifically intracellular bacteria that extensively manipulate host cell pathways, remains unknown. Here, we report that Salmonella infection induces P-body disassembly in a cell type-specific manner, and independently of previously characterized pathways such as inhibition of host cell RNA synthesis or microRNA-mediated gene silencing. We show that the Salmonella-induced P-body disassembly depends on the activation of the SPI-2 encoded type 3 secretion system, and that the secreted effector protein SpvB plays a major role in this process. P-body disruption is also induced by the related pathogen, Shigella flexneri, arguing that this might be a new mechanism by which intracellular bacterial pathogens subvert host cell function.

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