Epidemiological Survey of Human Rotavirus Serotypes and Electropherotypes in Young Children Admitted to Two Children's Hospitals in Northeast London from 1984 to 1990

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1991 Oct 1

PMID 1658035

Citations 20

Authors

J S Noel

G M Beards

W D Cubitt

Affiliations

Soon will be listed here.

Abstract

A retrospective and prospective survey was carried out to determine the relative frequency of rotavirus serotypes infecting children with diarrhea or vomiting or both who were admitted to the Hospitals for Sick Children in London during a 6-year period from 1984 to 1990. The results were compared with data for the same period from a study in Birmingham, United Kingdom. The serotype of rotaviruses infecting 1,019 children was ascertained by enzyme immunoassay with VP7-specific monoclonal antibodies. In London, serotype G1 accounted for 60% of the cases, serotype G4 accounted for 24%, serotype G2 accounted for 11%, G3 accounted for 3%, and coinfections accounted for 2%. Considerable differences in the relative prevalence of serotypes were seen when data from London and Birmingham were compared. A major shift from serotype G1 to G4 was observed in London in the 1989 to 1990 season, and a lesser shift was seen in Birmingham. Examination of the electrophoretic profiles of 611 rotaviruses from London showed that there were at least 108 different profiles. Continuous variation occurred throughout the 6-year period, and the same electropherotype never recurred once it had disappeared from the population. None of the electrophoretic profiles were characteristic of group B or group C rotaviruses. There was no evidence that any strain of rotavirus had become endemic in either of the children's hospitals in London.

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References

Aboudy Y, Shif I, Zilberstein I, Gotlieb-Stematsky T . Use of polyclonal and monoclonal antibodies and analysis of viral RNA in the detection of unusual group A human rotaviruses. J Med Virol. 1988; 25(3):351-9. DOI: 10.1002/jmv.1890250312. View

Georges-Courbot M, Beraud A, Beards G, Campbell A, Gonzalez J, Georges A . Subgroups, serotypes, and electrophoretypes of rotavirus isolated from children in Bangui, Central African Republic. J Clin Microbiol. 1988; 26(4):668-71. PMC: 266405. DOI: 10.1128/jcm.26.4.668-671.1988. View

Linhares A, Gabbay Y, Mascarenhas J, Freitas R, Flewett T, Beards G . Epidemiology of rotavirus subgroups and serotypes in Belem, Brazil: a three-year study. Ann Inst Pasteur Virol. 1988; 139(1):89-99. DOI: 10.1016/s0769-2617(88)80009-1. View

Heath R, Birch C, Gust I . Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11. J Gen Virol. 1986; 67 ( Pt 11):2455-66. DOI: 10.1099/0022-1317-67-11-2455. View

Hoshino Y, Sereno M, Midthun K, Flores J, Kapikian A, Chanock R . Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985; 82(24):8701-4. PMC: 391504. DOI: 10.1073/pnas.82.24.8701. View

Taniguchi K, Urasawa T, Morita Y, Greenberg H, URASAWA S . Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987; 155(6):1159-66. DOI: 10.1093/infdis/155.6.1159. View

Coulson B, Unicomb L, Pitson G, Bishop R . Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987; 25(3):509-15. PMC: 265970. DOI: 10.1128/jcm.25.3.509-515.1987. View

Nakagomi O, Nakagomi T, Hoshino Y, Flores J, Kapikian A . Genetic analysis of a human rotavirus that belongs to subgroup I but has an RNA pattern typical of subgroup II human rotaviruses. J Clin Microbiol. 1987; 25(7):1159-64. PMC: 269167. DOI: 10.1128/jcm.25.7.1159-1164.1987. View

Taniguchi K, Hoshino Y, Nishikawa K, Green K, Maloy W, Morita Y . Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988; 62(6):1870-4. PMC: 253268. DOI: 10.1128/JVI.62.6.1870-1874.1988. View

10.

Taniguchi K, Morita Y, Urasawa T, URASAWA S . Cross-reactive neutralization epitopes on VP3 of human rotavirus: analysis with monoclonal antibodies and antigenic variants. J Virol. 1987; 61(5):1726-30. PMC: 254165. DOI: 10.1128/JVI.61.5.1726-1730.1987. View

11.

Kobayashi N, Lintag I, Urasawa T, Taniguchi K, Saniel M, URASAWA S . Unusual human rotavirus strains having subgroup I specificity and "long" RNA electropherotype. Arch Virol. 1989; 109(1-2):11-23. DOI: 10.1007/BF01310514. View

12.

Beards G, Desselberger U, Flewett T . Temporal and geographical distributions of human rotavirus serotypes, 1983 to 1988. J Clin Microbiol. 1989; 27(12):2827-33. PMC: 267135. DOI: 10.1128/jcm.27.12.2827-2833.1989. View

13.

Nakagomi O, Oyamada H, Kuroki S, Kobayashi Y, Ohshima A, Nakagomi T . Molecular identification of a novel human rotavirus in relation to subgroup and electropherotype of genomic RNA. J Med Virol. 1989; 28(3):163-8. DOI: 10.1002/jmv.1890280311. View

14.

URASAWA S, Urasawa T, Taniguchi K, WAKASUGI F, Kobayashi N, Chiba S . Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis. 1989; 160(1):44-51. DOI: 10.1093/infdis/160.1.44. View

15.

Unicomb L, Coulson B, Bishop R . Experience with an enzyme immunoassay for serotyping human group A rotaviruses. J Clin Microbiol. 1989; 27(3):586-8. PMC: 267369. DOI: 10.1128/jcm.27.3.586-588.1989. View

16.

Zheng B, Lam W, Yan Y, Lo S, Lung M, Ng M . Direct identification of serotypes of natural human rotavirus isolates by hybridization using cDNA probes derived from segment 9 of the rotavirus genome. J Clin Microbiol. 1989; 27(3):552-7. PMC: 267356. DOI: 10.1128/jcm.27.3.552-557.1989. View

17.

Lanata C, Black R, del Aguila R, Gil A, Verastegui H, Gerna G . Protection of Peruvian children against rotavirus diarrhea of specific serotypes by one, two, or three doses of the RIT 4237 attenuated bovine rotavirus vaccine. J Infect Dis. 1989; 159(3):452-9. DOI: 10.1093/infdis/159.3.452. View

18.

Gerna G, Sarasini A, Zentilin L, di Matteo A, Miranda P, Parea M . Isolation in Europe of 69 M-like (serotype 8) human rotavirus strains with either subgroup I or II specificity and a long RNA electropherotype. Arch Virol. 1990; 112(1-2):27-40. DOI: 10.1007/BF01348983. View

19.

Gerna G, Sarasini A, Arista S, di Matteo A, Giovannelli L, Parea M . Prevalence of human rotavirus serotypes in some European countries 1981-1988. Scand J Infect Dis. 1990; 22(1):5-10. DOI: 10.3109/00365549009023112. View

20.

Greenberg H, McAULIFFE V, Valdesuso J, Wyatt R, Flores J, Kalica A . Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies. Infect Immun. 1983; 39(1):91-9. PMC: 347909. DOI: 10.1128/iai.39.1.91-99.1983. View