Serological Analysis of the Subgroup Protein of Rotavirus, Using Monoclonal Antibodies

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 Jan 1

PMID 6185436

Citations 154

Authors

H Greenberg

V McAULIFFE

J Valdesuso

R Wyatt

J Flores

A Kalica

Y Hoshino

N Singh

Affiliations

Soon will be listed here.

Abstract

Ten monoclones directed to the 42,000-dalton inner structural protein of rotavirus were analyzed. Eight monoclones reacted broadly with antigenic domains common to virtually all mammalian rotaviruses. Two monoclones had specificities similar or identical to previously characterized subgroup specificities. These subgroup monoclones were more efficient in detecting subgroup antigen than either hyperimmune or postinfection antisera. Using the subgroup monoclones, we determined that some animal as well as human rotavirus strains carry subgroup 2 specificity and that epizootic diarrhea of infant mice virus and turkey rotavirus are antigenically distinct from other mammalian rotavirus strains.

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