Experience with an Enzyme Immunoassay for Serotyping Human Group A Rotaviruses

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1989 Mar 1

PMID 2541171

Citations 23

Authors

L E Unicomb

B S Coulson

R F Bishop

Affiliations

Soon will be listed here.

Abstract

An enzyme immunoassay utilizing neutralizing monoclonal antibodies to VP7 of four human group A rotavirus serotypes successfully typed rotaviruses in 71.4% (568 of 796) of fecal specimens. Sensitivity was enhanced by using homologous capture and detector antibodies. Serotyping was most successful with specimens stored for less than 3 years and containing 10(4) or more particles per ml.

Citing Articles

Diversity of group A human rotavirus types circulating over a 4-year period in Madrid, Spain.

Sanchez-Fauquier A, Wilhelmi I, Colomina J, Cubero E, Roman E J Clin Microbiol. 2004; 42(4):1609-13.

PMID: 15071013 PMC: 387563. DOI: 10.1128/JCM.42.4.1609-1613.2004.

Antigenic and genetic characterization of serotype G2 human rotavirus strains from the African continent.

Page N, Steele A J Clin Microbiol. 2004; 42(2):595-600.

PMID: 14766822 PMC: 344437. DOI: 10.1128/JCM.42.2.595-600.2004.

Distribution of rotavirus VP4 genotypes and VP7 serotypes among nonhospitalized and hospitalized patients with gastroenteritis and patients with nosocomially acquired gastroenteritis in Austria.

Fruhwirth M, Brosl S, Ellemunter H, Rohwedder A, Mutz I J Clin Microbiol. 2000; 38(5):1804-6.

PMID: 10790103 PMC: 86593. DOI: 10.1128/JCM.38.5.1804-1806.2000.

Development, characterization, and diagnostic applications of monoclonal antibodies against bovine rotavirus.

Anderson J, Kapil S Clin Diagn Lab Immunol. 2000; 7(2):288-92.

PMID: 10702506 PMC: 95862. DOI: 10.1128/CDLI.7.2.288-292.2000.

Comparison of enzyme immunoassay and reverse transcriptase PCR for identification of serotype G9 rotaviruses.

Coulson B, Gentsch J, Das B, Bhan M, Glass R J Clin Microbiol. 1999; 37(10):3187-93.

PMID: 10488175 PMC: 85524. DOI: 10.1128/JCM.37.10.3187-3193.1999.

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10.

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