Ligation of Endogenous TRNA 3' Half Molecules to Their Corresponding 5' Halves Via 2'-phosphomonoester,3',5'-phosphodiester Bonds in Extracts of Chlamydomonas

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1983 Jan 1

PMID 16453445

Citations 14

Authors

K Tyc

Y Kikuchi

M Konarska

W Filipowicz

H J Gross

Affiliations

Soon will be listed here.

Abstract

tRNA preparations from Chlamydomonas and wheat germ contain small amounts of tRNA 5' halves and corresponding 3' halves. Incubation of cell-free extracts from the two sources with [gamma-P]ATP yielded 5'-P-labeled tRNA 3' halves which were joined to their corresponding 5' counterparts to form mature tRNA containing 2'-phosphomonoester,3', 5'-phosphodiester bonds. tRNA 3' halves labelled with T4 kinase were purified, sequenced and also joined to their 5' counterparts. It is proposed that these tRNA halves may be intermediates of the tRNA splicing process, and that the RNA kinase and ligase activities observed here are part of the tRNA splicing complex.

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