Genetic and Molecular Analysis of the Enhancer (En) Transposable Element System of Zea Mays

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1985 Jan 1

PMID 15915572

Citations 39

Authors

A Pereira

Z Schwarz-Sommer

A Gierl

I Bertram

P A Peterson

H Saedler

Affiliations

Soon will be listed here.

Abstract

A newly isolated, unstable mutation wx-844::En-1 of Zea mays was proven to be caused by the insertion of the autonomous transposable element En into the Waxy (Wx) gene. Molecular analysis revealed that En-1 is 8.4 kb long, has a 13-bp long perfect inverted repeat at its termini and generates a 3-bp target site duplication. En-1 is integrated into an intron located approximately in the middle of the transcribed region of the Wx gene. Structural evidence is presented indicating that a receptor component (Inhibitor) can arise by internal deletion of an autonomous En element.

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Molecular analysis of paramutant plants of Antirrhinum majus and the involvement of transposable elements.

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References

Peterson P . The Pale Green Mutable System in Maize. Genetics. 1960; 45(1):115-33. PMC: 1209998. DOI: 10.1093/genetics/45.1.115. View

Frischauf A, Lehrach H, Poustka A, Murray N . Lambda replacement vectors carrying polylinker sequences. J Mol Biol. 1983; 170(4):827-42. DOI: 10.1016/s0022-2836(83)80190-9. View

Weck E, Courage U, Doring H, Fedoroff N, Starlinger P . Analysis of sh-m6233, a mutation induced by the transposable element Ds in the sucrose synthase gene of Zea mays. EMBO J. 1984; 3(8):1713-6. PMC: 557586. DOI: 10.1002/j.1460-2075.1984.tb02036.x. View

Fedoroff N, Wessler S, Shure M . Isolation of the transposable maize controlling elements Ac and Ds. Cell. 1983; 35(1):235-42. DOI: 10.1016/0092-8674(83)90226-x. View

Davis R, HYMAN R . A study in evolution: the DNA base sequence homology between coliphages T7 and T3. J Mol Biol. 1971; 62(2):287-301. DOI: 10.1016/0022-2836(71)90428-1. View

Fedoroff N, Furtek D, Nelson O . Cloning of the bronze locus in maize by a simple and generalizable procedure using the transposable controlling element Activator (Ac). Proc Natl Acad Sci U S A. 1984; 81(12):3825-9. PMC: 345313. DOI: 10.1073/pnas.81.12.3825. View

Rhoades M . Meiosis in maize. J Hered. 1950; 41(3):59-67. DOI: 10.1093/oxfordjournals.jhered.a106089. View

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

10.

Bonas U, Sommer H, Saedler H . The 17-kb Tam1 element of Antirrhinum majus induces a 3-bp duplication upon integration into the chalcone synthase gene. EMBO J. 1984; 3(5):1015-9. PMC: 557465. DOI: 10.1002/j.1460-2075.1984.tb01921.x. View

11.

Benton W, Davis R . Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977; 196(4286):180-2. DOI: 10.1126/science.322279. View

12.

Yamamoto K, Alberts B, Benzinger R, Lawhorne L, Treiber G . Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970; 40(3):734-44. DOI: 10.1016/0042-6822(70)90218-7. View

13.

Lis J, Schleif R . Size fractionation of double-stranded DNA by precipitation with polyethylene glycol. Nucleic Acids Res. 1975; 2(3):383-9. PMC: 342844. DOI: 10.1093/nar/2.3.383. View

14.

Pohlman R, Fedoroff N, Messing J . The nucleotide sequence of the maize controlling element Activator. Cell. 1984; 37(2):635-43. DOI: 10.1016/0092-8674(84)90395-7. View

15.

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

16.

Shure M, Wessler S, Fedoroff N . Molecular identification and isolation of the Waxy locus in maize. Cell. 1983; 35(1):225-33. DOI: 10.1016/0092-8674(83)90225-8. View

17.

Nelson O . The WAXY Locus in Maize. II. the Location of the Controlling Element Alleles. Genetics. 1968; 60(3):507-24. PMC: 1212058. DOI: 10.1093/genetics/60.3.507. View

18.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

19.

Schwarz-Sommer Z, Gierl A, Klosgen R, Wienand U, Peterson P, Saedler H . The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx allele of Zea mays. EMBO J. 1984; 3(5):1021-8. PMC: 557466. DOI: 10.1002/j.1460-2075.1984.tb01922.x. View

20.

Peterson P . Mutable a(1) of the En System in Maize. Genetics. 1961; 46(7):759-71. PMC: 1210238. DOI: 10.1093/genetics/46.7.759. View