Molecular Analysis of the En/Spm Transposable Element System of Zea Mays

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1986 May 1

PMID 15957213

Citations 98

Authors

A Pereira

H Cuypers

A Gierl

Z Schwarz-Sommer

H Saedler

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of the autonomous transposable element En-1 isolated from the wx-844::En-1 allele has been determined. En-1 is 8287 bp long. The structure of the mosaic gene 1, coding for the major En transcript, has been established. The promoter gene 1 is located in the highly structured left end of the element and the gene spans almost the entire length of En-1. The first intron of gene 1 is 4434 nucleotides long and contains two large open reading frames, 2714 bp and 761 bp in size, which hybridize to minor RNA species in Northern blot experiments.

Citing Articles

A CACTA-like transposon in the Anthocyanidin synthase 1 (Ans-1) gene is responsible for apricot fruit colour in the raspberry (Rubus idaeus) cultivar 'Varnes'.

Sargent D, Buti M, Martens S, Pugliesi C, Aaby K, Roen D PLoS One. 2025; 20(2):e0318692.

PMID: 39899506 PMC: 11790086. DOI: 10.1371/journal.pone.0318692.

Maize kernel development.

Dai D, Ma Z, Song R Mol Breed. 2023; 41(1):2.

PMID: 37309525 PMC: 10231577. DOI: 10.1007/s11032-020-01195-9.

Androgenic-Induced Transposable Elements Dependent Sequence Variation in Barley.

Orlowska R, Pachota K, Dynkowska W, Niedziela A, Bednarek P Int J Mol Sci. 2021; 22(13).

PMID: 34202586 PMC: 8268840. DOI: 10.3390/ijms22136783.

Isolating an active and inactive CACTA transposon from lettuce color mutants and characterizing their family.

Gurdon C, Kozik A, Tao R, Poulev A, Armas I, Michelmore R Plant Physiol. 2021; 186(2):929-944.

PMID: 33768232 PMC: 8195511. DOI: 10.1093/plphys/kiab143.

On the Trail of : Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome.

Ventimiglia M, Pugliesi C, Vangelisti A, Usai G, Giordani T, Natali L Int J Mol Sci. 2020; 21(6).

PMID: 32188063 PMC: 7139988. DOI: 10.3390/ijms21062021.

References

Paz-Ares J, Wienand U, Peterson P, Saedler H . Molecular cloning of the c locus of Zea mays: a locus regulating the anthocyanin pathway. EMBO J. 1986; 5(5):829-33. PMC: 1166870. DOI: 10.1002/j.1460-2075.1986.tb04291.x. View

Bird A . DNA methylation and the frequency of CpG in animal DNA. Nucleic Acids Res. 1980; 8(7):1499-504. PMC: 324012. DOI: 10.1093/nar/8.7.1499. View

Land H, Grez M, Hauser H, Lindenmaier W, Schutz G . 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981; 9(10):2251-66. PMC: 326843. DOI: 10.1093/nar/9.10.2251. View

Pereira A, Schwarz-Sommer Z, Gierl A, Bertram I, Peterson P, Saedler H . Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays. EMBO J. 1985; 4(1):17-23. PMC: 554145. DOI: 10.1002/j.1460-2075.1985.tb02311.x. View

Peterson P . Instability among the components of a regulatory element transposon in maize. Cold Spring Harb Symp Quant Biol. 1981; 45 Pt 2:447-55. DOI: 10.1101/sqb.1981.045.01.059. View

Gierl A, Schwarz-Sommer Z, Saedler H . Molecular interactions between the components of the En-I transposable element system of Zea mays. EMBO J. 1985; 4(3):579-83. PMC: 554228. DOI: 10.1002/j.1460-2075.1985.tb03669.x. View

OReilly C, Shepherd N, Pereira A, Schwarz-Sommer Z, Bertram I, Robertson D . Molecular cloning of the a1 locus of Zea mays using the transposable elements En and Mu1. EMBO J. 1985; 4(4):877-82. PMC: 554274. DOI: 10.1002/j.1460-2075.1985.tb03713.x. View

Bennetzen J, Swanson J, Taylor W, Freeling M . DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proc Natl Acad Sci U S A. 1984; 81(13):4125-8. PMC: 345381. DOI: 10.1073/pnas.81.13.4125. View

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

10.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

11.

Kozak M . Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucleic Acids Res. 1984; 12(2):857-72. PMC: 318541. DOI: 10.1093/nar/12.2.857. View

12.

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

13.

Weaver R, Weissmann C . Mapping of RNA by a modification of the Berk-Sharp procedure: the 5' termini of 15 S beta-globin mRNA precursor and mature 10 s beta-globin mRNA have identical map coordinates. Nucleic Acids Res. 1979; 7(5):1175-93. PMC: 342295. DOI: 10.1093/nar/7.5.1175. View

14.

Pohlman R, Fedoroff N, Messing J . The nucleotide sequence of the maize controlling element Activator. Cell. 1984; 37(2):635-43. DOI: 10.1016/0092-8674(84)90395-7. View

15.

Nevers P, Saedler H . Transposable genetic elements as agents of gene instability and chromosomal rearrangements. Nature. 1977; 268(5616):109-15. DOI: 10.1038/268109a0. View

16.

Schwarz-Sommer Z, Gierl A, Berndtgen R, Saedler H . Sequence comparison of 'states' of a1-m1 suggests a model of Spm (En) action. EMBO J. 1985; 4(10):2439-43. PMC: 554526. DOI: 10.1002/j.1460-2075.1985.tb03953.x. View

17.

Lapeyre B, Amalric F . A powerful method for the preparation of cDNA libraries: isolation of cDNA encoding a 100-kDal nucleolar protein. Gene. 1985; 37(1-3):215-20. DOI: 10.1016/0378-1119(85)90275-6. View

18.

Vieira J, Messing J . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985; 33(1):103-19. DOI: 10.1016/0378-1119(85)90120-9. View

19.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

20.

Schwarz-Sommer Z, Gierl A, Klosgen R, Wienand U, Peterson P, Saedler H . The Spm (En) transposable element controls the excision of a 2-kb DNA insert at the wx allele of Zea mays. EMBO J. 1984; 3(5):1021-8. PMC: 557466. DOI: 10.1002/j.1460-2075.1984.tb01922.x. View