The Inveterate Wanderer: Study of Enhancer Wandering on Chromosome 3 in Maize

Overview

Journal Theor Appl Genet

Publisher Springer

Specialty Genetics

Date 2013 Oct 29

PMID 24162212

Authors

B S Seo

P A Peterson

Affiliations

Soon will be listed here.

Abstract

The transposition of the maize transposable element Enhancer (En) had been focused on one chromosome 3 for several generations. From the a1-m(Au) allele with an autonomous En, a new En reporter allele a1-m(r)3927-1, was isolated that undergoes very infrequent and late excision events, producing one or two small spots in the aleurone. This allele is seriously impaired in its capacity to excise. Coincident with the origin of this allele, an En was located at a site close to the a1 locus. From this initial insertion site, the movement of this En was followed for three to four generations in 974 families with a higher transposition rate of this En (50% of the testcross progeny) than that found in a previous study of En transposition. This is the first case reported where a particular En was followed for more than three generations. The higher rate of wanderings of this En along the same chromosome led to the term 'vagabond' En (En (vag) ). Genetic evidence that En may transpose from a replicated donor site to an unreplicated site is provided. Speculative mechanisms on the origin of a1-m(r)3927-1 and En (vag) are discussed.

References

Van Schaik N, Brink R . Transpositions of Modulator, a Component of the Variegated Pericarp Allele in Maize. Genetics. 1959; 44(4):725-38. PMC: 1209976. DOI: 10.1093/genetics/44.4.725. View

Chomet P, Wessler S, Dellaporta S . Inactivation of the maize transposable element Activator (Ac) is associated with its DNA modification. EMBO J. 1987; 6(2):295-302. PMC: 553394. DOI: 10.1002/j.1460-2075.1987.tb04753.x. View

Greenblatt I . A chromosome replication pattern deduced from pericarp phenotypes resulting from movements of the transposable element, modulator, in maize. Genetics. 1984; 108(2):471-85. PMC: 1202418. DOI: 10.1093/genetics/108.2.471. View

Athma P, Grotewold E, Peterson T . Insertional mutagenesis of the maize P gene by intragenic transposition of Ac. Genetics. 1992; 131(1):199-209. PMC: 1204954. DOI: 10.1093/genetics/131.1.199. View

Dooner H, Belachew A . Transposition Pattern of the Maize Element Ac from the Bz-M2(ac) Allele. Genetics. 1989; 122(2):447-57. PMC: 1203716. DOI: 10.1093/genetics/122.2.447. View

Schiefelbein J, Raboy V, Fedoroff N, Nelson Jr O . Deletions within a defective suppressor-mutator element in maize affect the frequency and developmental timing of its excision from the bronze locus. Proc Natl Acad Sci U S A. 1985; 82(14):4783-7. PMC: 390989. DOI: 10.1073/pnas.82.14.4783. View

Peterson P . The En mutable system in maize : III. Transposition associated with mutational events. Theor Appl Genet. 2014; 40(8):367-77. DOI: 10.1007/BF00285416. View

McCLINTOCK B . Chromosome organization and genic expression. Cold Spring Harb Symp Quant Biol. 1951; 16:13-47. DOI: 10.1101/sqb.1951.016.01.004. View

Chen J, Greenblatt I, Dellaporta S . Transposition of Ac from the P locus of maize into unreplicated chromosomal sites. Genetics. 1987; 117(1):109-16. PMC: 1203179. DOI: 10.1093/genetics/117.1.109. View

10.

Dash S, Peterson P . Frequent loss of the En transposable element after excision and its relation to chromosome replication in maize (Zea mays L.). Genetics. 1994; 136(2):653-71. PMC: 1205815. DOI: 10.1093/genetics/136.2.653. View

11.

Grant S, Hardenack S, Trentmann S, Saedler H . Functional cis-element sequence requirements for suppression of gene expression by the TNPA protein of the Zea mays transposon En/Spm. Mol Gen Genet. 1993; 241(1-2):153-60. DOI: 10.1007/BF00280212. View

12.

Schwarz-Sommer Z, Gierl A, Cuypers H, Peterson P, Saedler H . Plant transposable elements generate the DNA sequence diversity needed in evolution. EMBO J. 1985; 4(3):591-7. PMC: 554230. DOI: 10.1002/j.1460-2075.1985.tb03671.x. View

13.

Osborne B, Corr C, Prince J, Hehl R, Tanksley S, McCormick S . Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome. Genetics. 1991; 129(3):833-44. PMC: 1204750. DOI: 10.1093/genetics/129.3.833. View

14.

Tower J, Karpen G, Craig N, Spradling A . Preferential transposition of Drosophila P elements to nearby chromosomal sites. Genetics. 1993; 133(2):347-59. PMC: 1205324. DOI: 10.1093/genetics/133.2.347. View

15.

Bancroft I, Dean C . Transposition pattern of the maize element Ds in Arabidopsis thaliana. Genetics. 1993; 134(4):1221-9. PMC: 1205589. DOI: 10.1093/genetics/134.4.1221. View

16.

Greenblatt I, Brink R . Twin Mutations in Medium Variegated Pericarp Maize. Genetics. 1962; 47(4):489-501. PMC: 1210347. DOI: 10.1093/genetics/47.4.489. View

17.

Raboy V, Kim H, Schiefelbein J, Nelson Jr O . Deletions in a dspm insert in a maize bronze-1 allele alter RNA processing and gene expression. Genetics. 1989; 122(3):695-703. PMC: 1203743. DOI: 10.1093/genetics/122.3.695. View

18.

Saedler H, Nevers P . Transposition in plants: a molecular model. EMBO J. 1985; 4(3):585-90. PMC: 554229. DOI: 10.1002/j.1460-2075.1985.tb03670.x. View

19.

Dooner H, Keller J, Harper E, Ralston E . Variable Patterns of Transposition of the Maize Element Activator in Tobacco. Plant Cell. 1991; 3(5):473-482. PMC: 160015. DOI: 10.1105/tpc.3.5.473. View

20.

Brink R, Nilan R . The Relation between Light Variegated and Medium Variegated Pericarp in Maize. Genetics. 1952; 37(5):519-44. PMC: 1209573. DOI: 10.1093/genetics/37.5.519. View