Evaluation of the Abilities of Three Diagnostic Tests Based on the Polymerase Chain Reaction to Detect Mycobacterium Paratuberculosis in Cattle: Application in a Control Program

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 May 1

PMID 1583122

Citations 20

Authors

J W van der Giessen

R M Haring

E Vauclare

A Eger

J Haagsma

B A van der Zeijst

Affiliations

Soon will be listed here.

Abstract

Three assays for the specific detection of Mycobacterium paratuberculosis by dot spot hybridization of polymerase chain reaction products were applied to fecal samples of dairy cattle. The first two tests used polymerase chain reaction primers and a DNA probe derived from M. paratuberculosis-specific sequences of the 16S rRNA gene and insertion element IS900, respectively. These two tests were carried out on spiked fecal samples to determine the detection limits. The 16S rRNA test was able to detect 10(7) bacteria per g of feces, and the IS900 test detected 10(4) to 10(5) per g of feces. Next, we studied the usefulness of these tests in a control program for paratuberculosis. Therefore, the tests and a third, commercially available, test (IDEXX Corp.) were used twice with an interval of 3 months on fecal samples of 87 cows from two dairy herds with a history of Johne's disease. We compared the results of these tests with those of culturing. This showed that the tests are specific but that the sensitivity ranged from 3 to 23%. Further improvement of the sensitivity is needed before the tests can be used in a control program to eradicate Johne's disease.

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References

Murray A, Moriarty K, Scott D . A cloned DNA probe for the detection of Mycobacterium paratuberculosis. N Z Vet J. 1989; 37(2):47-50. DOI: 10.1080/00480169.1989.35556. View

Beerwerth W . [The culture of mycobacteria from feces of domestic animalsand their significance for epidemiology and control of tuberculosis]. Prax Pneumol. 1967; 21(4):189-202. View

Collins M, Sockett D, Ridge S, Cox J . Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease. J Clin Microbiol. 1991; 29(2):272-6. PMC: 269752. DOI: 10.1128/jcm.29.2.272-276.1991. View

Milner A, Mack W, Coates K, Hill J, Gill I, Sheldrick P . The sensitivity and specificity of a modified ELISA for the diagnosis of Johne's disease from a field trial in cattle. Vet Microbiol. 1990; 25(2-3):193-8. DOI: 10.1016/0378-1135(90)90076-8. View

Colgrove G, Thoen C, Blackburn B, Murphy C . Paratuberculosis in cattle: a comparison of three serologic tests with results of fecal culture. Vet Microbiol. 1989; 19(2):183-7. DOI: 10.1016/0378-1135(89)90083-7. View

Hurley S, Splitter G, Welch R . Development of a diagnostic test for Johne's disease using a DNA hybridization probe. J Clin Microbiol. 1989; 27(7):1582-7. PMC: 267619. DOI: 10.1128/jcm.27.7.1582-1587.1989. View

McFadden J, Butcher P, Thompson J, Chiodini R, Hermon-Taylor J . The use of DNA probes identifying restriction-fragment-length polymorphisms to examine the Mycobacterium avium complex. Mol Microbiol. 1987; 1(3):283-91. DOI: 10.1111/j.1365-2958.1987.tb01934.x. View

Benedictus G, Dijkhuizen A, Stelwagen J . Economic losses due to paratuberculosis in dairy cattle. Vet Rec. 1987; 121(7):142-6. DOI: 10.1136/vr.121.7.142. View

Vary P, Andersen P, Green E, Hermon-Taylor J, McFadden J . Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol. 1990; 28(5):933-7. PMC: 267840. DOI: 10.1128/jcm.28.5.933-937.1990. View

10.

Jorgensen J . An improved medium for culture of Mycobacterium paratuberculosis from bovine faeces. Acta Vet Scand. 1982; 23(3):325-35. PMC: 8295813. View

11.

Chiodini R, Van Kruiningen H, MERKAL R . Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet. 1984; 74(3):218-62. View

12.

Wilesmith J . Johne's disease: a retrospective study of vaccinated herds in Great Britain. Br Vet J. 1982; 138(4):321-31. DOI: 10.1016/s0007-1935(17)31037-0. View

13.

de Lisle G, Samagh B, Duncan J . Bovine paratuberculosis II. A comparison of fecal culture and the antibody response. Can J Comp Med. 1980; 44(2):183-91. PMC: 1320055. View

14.

Larsen A, Moyle A, Himes E . Experimental vaccination of cattle against paratuberculosis (Johne's disease) with killed bacterial vaccines: a controlled field study. Am J Vet Res. 1978; 39(1):65-9. View

15.

Goudswaard J, Gilmour N, Dijkstra R, Van Beek J . Diagnosis of Johne's disease in cattle: a comparison of five serological tests under field conditions. Vet Rec. 1976; 98(23):461-2. DOI: 10.1136/vr.98.23.461. View

16.

MERKAL R, Larsen A, Kopecky K . Comparison of examination and test methods for early detection of paratuberculous cattle. Am J Vet Res. 1968; 29(8):1533-8. View

17.

van der Giessen J, Eger A, Haagsma J, Haring R, Gaastra W, van der Zeijst B . Amplification of 16S rRNA sequences to detect Mycobacterium paratuberculosis. J Med Microbiol. 1992; 36(4):255-63. DOI: 10.1099/00222615-36-4-255. View