Rapid Detection and Identification of Mycobacterium Avium by Amplification of 16S RRNA Sequences

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1993 Sep 1

PMID 7691877

Citations 3

Authors

J W van der Giessen

A Eger

J Haagsma

B A van der Zeijst

Affiliations

Soon will be listed here.

Abstract

An assay that is based on the amplification of 16S rRNA sequences and that was initially developed to detect Mycobacterium paratuberculosis in cattle was used to test 20 serotypes of the Mycobacterium avium complex (MAC) and atypical mycobacterial species not belonging to MAC. Only serotypes 1 to 6 and 8 to 11, designated M. avium, were detected by the assay, indicating that it can be used for the rapid detection and identification of M. avium. The results of the assay for clinical samples from animals suspected of having mycobacterial infections indicated that it can also be used directly on clinical samples.

Citing Articles

Differentiating host-associated variants of Mycobacterium avium by PCR for detection of large sequence polymorphisms.

Semret M, Turenne C, de Haas P, Collins D, Behr M J Clin Microbiol. 2006; 44(3):881-7.

PMID: 16517871 PMC: 1393138. DOI: 10.1128/JCM.44.3.881-887.2006.

Relationship between IS901 in the Mycobacterium avium complex strains isolated from birds, animals, humans, and the environment and virulence for poultry.

Pavlik I, Svastova P, Bartl J, Dvorska L, Rychlik I Clin Diagn Lab Immunol. 2000; 7(2):212-7.

PMID: 10702495 PMC: 95851. DOI: 10.1128/CDLI.7.2.212-217.2000.

Rapid differentiation of Mycobacterium avium and M. paratuberculosis by PCR and restriction enzyme analysis.

Eriks I, Munck K, Besser T, Cantor G, Kapur V J Clin Microbiol. 1996; 34(3):734-7.

PMID: 8904449 PMC: 228881. DOI: 10.1128/jcm.34.3.734-737.1996.

References

Barnes P, Bloch A, Davidson P, Snider Jr D . Tuberculosis in patients with human immunodeficiency virus infection. N Engl J Med. 1991; 324(23):1644-50. DOI: 10.1056/NEJM199106063242307. View

Drake T, Hindler J, Berlin O, Bruckner D . Rapid identification of Mycobacterium avium complex in culture using DNA probes. J Clin Microbiol. 1987; 25(8):1442-5. PMC: 269243. DOI: 10.1128/jcm.25.8.1442-1445.1987. View

Kunze Z, Wall S, Appelberg R, Silva M, Portaels F, McFadden J . IS901, a new member of a widespread class of atypical insertion sequences, is associated with pathogenicity in Mycobacterium avium. Mol Microbiol. 1991; 5(9):2265-72. DOI: 10.1111/j.1365-2958.1991.tb02157.x. View

Tsang A, Denner J, Brennan P, McClatchy J . Clinical and epidemiological importance of typing of Mycobacterium avium complex isolates. J Clin Microbiol. 1992; 30(2):479-84. PMC: 265081. DOI: 10.1128/jcm.30.2.479-484.1992. View

van der Giessen J, Eger A, Haagsma J, Haring R, Gaastra W, van der Zeijst B . Amplification of 16S rRNA sequences to detect Mycobacterium paratuberculosis. J Med Microbiol. 1992; 36(4):255-63. DOI: 10.1099/00222615-36-4-255. View

van der Giessen J, Haring R, Vauclare E, Eger A, Haagsma J, van der Zeijst B . Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol. 1992; 30(5):1216-9. PMC: 265253. DOI: 10.1128/jcm.30.5.1216-1219.1992. View

WOLINSKY E . Nontuberculous mycobacteria and associated diseases. Am Rev Respir Dis. 1979; 119(1):107-59. DOI: 10.1164/arrd.1979.119.1.107. View

Thoen C, KARLSON A, Himes E . Mycobacterial infections in animals. Rev Infect Dis. 1981; 3(5):960-72. DOI: 10.1093/clinids/3.5.960. View

Armstrong D, Gold J, Dryjanski J, Whimbey E, Polsky B, Hawkins C . Treatment of infections in patients with the acquired immunodeficiency syndrome. Ann Intern Med. 1985; 103(5):738-43. DOI: 10.7326/0003-4819-103-5-738. View

10.

Woese C . Bacterial evolution. Microbiol Rev. 1987; 51(2):221-71. PMC: 373105. DOI: 10.1128/mr.51.2.221-271.1987. View

11.

Smit T, Eger A, Haagsma J, Bakhuizen T . Avian tuberculosis in wild birds in the Netherlands. J Wildl Dis. 1987; 23(3):485-7. DOI: 10.7589/0090-3558-23.3.485. View

12.

Saxegaard F, Baess I . Relationship between Mycobacterium avium, Mycobacterium paratuberculosis and "wood pigeon mycobacteria". Determinations by DNA-DNA hybridization. APMIS. 1988; 96(1):37-42. View

13.

Musial C, Tice L, Stockman L, Roberts G . Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex. J Clin Microbiol. 1988; 26(10):2120-3. PMC: 266828. DOI: 10.1128/jcm.26.10.2120-2123.1988. View

14.

Prince D, Peterson D, Steiner R, Gottlieb J, Scott R, ISRAEL H . Infection with Mycobacterium avium complex in patients without predisposing conditions. N Engl J Med. 1989; 321(13):863-8. DOI: 10.1056/NEJM198909283211304. View

15.

Thorel M, Varnerot A, Gicquel B . DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis. J Clin Microbiol. 1989; 27(12):2823-6. PMC: 267134. DOI: 10.1128/jcm.27.12.2823-2826.1989. View

16.

Vary P, Andersen P, Green E, Hermon-Taylor J, McFadden J . Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol. 1990; 28(5):933-7. PMC: 267840. DOI: 10.1128/jcm.28.5.933-937.1990. View

17.

. Diagnosis and treatment of disease caused by nontuberculous mycobacteria. Am Rev Respir Dis. 1990; 142(4):940-53. DOI: 10.1164/ajrccm/142.4.940. View

18.

Boddinghaus B, Wolters J, Heikens W, Bottger E . Phylogenetic analysis and identification of different serovars of Mycobacterium intracellulare at the molecular level. FEMS Microbiol Lett. 1990; 58(2):197-203. DOI: 10.1111/j.1574-6968.1990.tb13978.x. View

19.

Rogall T, Wolters J, Flohr T, Bottger E . Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990; 40(4):323-30. DOI: 10.1099/00207713-40-4-323. View

20.

McAdam R, Hermans P, van Soolingen D, Zainuddin Z, Catty D, van Embden J . Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family. Mol Microbiol. 1990; 4(9):1607-13. DOI: 10.1111/j.1365-2958.1990.tb02073.x. View