Detection of Mycobacterium Paratuberculosis in Formalin-fixed Paraffin-embedded Tissues by the Polymerase Chain Reaction

Overview

Journal Can J Vet Res

Specialty Veterinary Medicine

Date 1996 Apr 1

PMID 8785716

Citations 3

Authors

Y Plante

B W Remenda

B J Chelack

D M Haines

Affiliations

Soon will be listed here.

Abstract

The polymerase chain reaction (PCR) utilizing primers specific for the IS900 sequence of Mycobacterium paratuberculosis was applied to tissue sections of formalin-fixed, paraffin-embedded ileum from cattle with Johne's disease and the results compared to those obtained with acid-fast (Ziehl-Neelsen) and immunohistochemical staining. The PCR was positive in 19/21 tissues (90%) while Ziehl-Neelsen staining was positive in 18/21 (86%) and immunohistochemical staining in 21/21 (100%). The Ziehl-Neelsen and immunohistochemical stains are not, however, specific for M. paratuberculosis. The PCR for detection of M. paratuberculosis using the IS900 sequence is a specific and relatively sensitive method for confirmation of Johne's disease and its application to formalin-fixed, paraffin-embedded tissues may be useful for confirmation of dubious cases, for retrospective studies and for epidemiological analyses.

Citing Articles

Detection of caprine paratuberculosis (Johne's disease) in pre- and post-vaccinated herds: morphological diagnosis, lesion grading, and bacterial identification.

Stefanova E, Sierra E, Fernandez A, Quesada-Canales O, Paz-Sanchez Y, Colom-Rivero A Front Vet Sci. 2024; 11:1395928.

PMID: 39144076 PMC: 11322454. DOI: 10.3389/fvets.2024.1395928.

Duplex PCR for differential identification of Mycobacterium bovis, M. avium, and M. avium subsp. paratuberculosis in formalin- fixed paraffin-embedded tissues from cattle.

Coetsier C, Vannuffel P, Blondeel N, Denef J, Cocito C, Gala J J Clin Microbiol. 2000; 38(8):3048-54.

PMID: 10921976 PMC: 87183. DOI: 10.1128/JCM.38.8.3048-3054.2000.

Detection of Mycobacterium avium subsp. paratuberculosis in infected tissues by new species-specific immunohistological procedures.

Coetsier C, Havaux X, Mattelard F, Sadatte S, Cormont F, Buergelt K Clin Diagn Lab Immunol. 1998; 5(4):446-51.

PMID: 9665946 PMC: 95597. DOI: 10.1128/CDLI.5.4.446-451.1998.

References

Collins D, Gabric D, de Lisle G . Identification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization. J Clin Microbiol. 1990; 28(7):1591-6. PMC: 267994. DOI: 10.1128/jcm.28.7.1591-1596.1990. View

Vary P, Andersen P, Green E, Hermon-Taylor J, McFadden J . Use of highly specific DNA probes and the polymerase chain reaction to detect Mycobacterium paratuberculosis in Johne's disease. J Clin Microbiol. 1990; 28(5):933-7. PMC: 267840. DOI: 10.1128/jcm.28.5.933-937.1990. View

Wiley E, Mulhollan T, Beck B, Tyndall J, Freeman R . Polyclonal antibodies raised against Bacillus Calmette-Guerin, Mycobacterium duvalii, and Mycobacterium paratuberculosis used to detect mycobacteria in tissue with the use of immunohistochemical techniques. Am J Clin Pathol. 1990; 94(3):307-12. DOI: 10.1093/ajcp/94.3.307. View

Ambrosio R, Harris Y, Huchzermeyer H . A DNA probe for the detection of Mycobacterium paratuberculosis. Vet Microbiol. 1991; 26(1-2):87-93. DOI: 10.1016/0378-1135(91)90044-g. View

Moss M, Green E, Tizard M, Malik Z, Hermon-Taylor J . Specific detection of Mycobacterium paratuberculosis by DNA hybridisation with a fragment of the insertion element IS900. Gut. 1991; 32(4):395-8. PMC: 1379078. DOI: 10.1136/gut.32.4.395. View

Haines D, Chelack B . Technical considerations for developing enzyme immunohistochemical staining procedures on formalin-fixed paraffin-embedded tissues for diagnostic pathology. J Vet Diagn Invest. 1991; 3(1):101-12. DOI: 10.1177/104063879100300128. View

de Wit M, Faber W, Krieg S, Douglas J, Lucas S, Montreewasuwat N . Application of a polymerase chain reaction for the detection of Mycobacterium leprae in skin tissues. J Clin Microbiol. 1991; 29(5):906-10. PMC: 269906. DOI: 10.1128/jcm.29.5.906-910.1991. View

Aznar C, Chureau C, Nguyen S, Pierre C, Bartoli M, Bonete R . Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation. Lancet. 1991; 338(8763):364-6. DOI: 10.1016/0140-6736(91)90492-8. View

Whipple D, Kapke P, Andersen P . Comparison of a commercial DNA probe test and three cultivation procedures for detection of Mycobacterium paratuberculosis in bovine feces. J Vet Diagn Invest. 1992; 4(1):23-7. DOI: 10.1177/104063879200400106. View

10.

van der Giessen J, Eger A, Haagsma J, Haring R, Gaastra W, van der Zeijst B . Amplification of 16S rRNA sequences to detect Mycobacterium paratuberculosis. J Med Microbiol. 1992; 36(4):255-63. DOI: 10.1099/00222615-36-4-255. View

11.

van der Giessen J, Haring R, Vauclare E, Eger A, Haagsma J, van der Zeijst B . Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol. 1992; 30(5):1216-9. PMC: 265253. DOI: 10.1128/jcm.30.5.1216-1219.1992. View

12.

Sockett D, Carr D, Collins M . Evaluation of conventional and radiometric fecal culture and a commercial DNA probe for diagnosis of Mycobacterium paratuberculosis infections in cattle. Can J Vet Res. 1992; 56(2):148-53. PMC: 1263523. View

13.

Avery B, Jorgensen C, Madison V, Greve T . Morphological development and sex of bovine in vitro-fertilized embryos. Mol Reprod Dev. 1992; 32(3):265-70. DOI: 10.1002/mrd.1080320312. View

14.

Fiallo P, Williams D, Chan G, Gillis T . Effects of fixation on polymerase chain reaction detection of Mycobacterium leprae. J Clin Microbiol. 1992; 30(12):3095-8. PMC: 270594. DOI: 10.1128/jcm.30.12.3095-3098.1992. View

15.

Collins D, Stephens D, de Lisle G . Comparison of polymerase chain reaction tests and faecal culture for detecting Mycobacterium paratuberculosis in bovine faeces. Vet Microbiol. 1993; 36(3-4):289-99. DOI: 10.1016/0378-1135(93)90095-o. View

16.

Challans J, Stevenson K, Reid H, Sharp J . A rapid method for the extraction and detection of Mycobacterium avium subspecies paratuberculosis from clinical specimens. Vet Rec. 1994; 134(4):95-6. DOI: 10.1136/vr.134.4.95. View

17.

Skowronski J, Plucienniczak A, Bednarek A, Jaworski J . Bovine 1.709 satellite. Recombination hotspots and dispersed repeated sequences. J Mol Biol. 1984; 177(3):399-416. DOI: 10.1016/0022-2836(84)90292-4. View

18.

Thorel M . Review of the occurrence of mycobactin dependence among mycobacteria species. Ann Rech Vet. 1984; 15(3):405-9. View

19.

Chiodini R . Biochemical characteristics of various strains of Mycobacterium paratuberculosis. Am J Vet Res. 1986; 47(7):1442-5. View

20.

Thorns C, Morris J . Shared epitopes between mycobacteria and other microorganisms. Res Vet Sci. 1986; 41(2):275-6. View