Identification and Characterization of Mycobacterium Paratuberculosis Recombinant Proteins Expressed in E. Coli

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 1994 Sep 1

PMID 7765093

Citations 10

Authors

S A Naser

L Engstrand

C Y Hachem

D Y Graham

Affiliations

Soon will be listed here.

Abstract

Mycobacterium paratuberculosis is the causative agent of Johne's disease, a chronic enteritis in ruminants, and it has also been isolated and identified from patients with Crohn's disease, an inflammatory bowel disease. The control of Johne's disease has been hampered by the lack of a reliable diagnostic test because of the large degree of antigenic cross-reactivity between mycobacterial and non-mycobacterial species. To help identify specific antigen(s) or epitope(s), an M. paratuberculosis expression library was screened with antibodies and DNA probes. In total, 54 clones were randomly picked, purified, and characterized by DNA probes and monoclonal antibodies with known specificity to individual mycobacterial antigens. Four clones carrying the heat shock protein 65K-, two representing the secreted protein 32K-, three representing the 21K-, and 20 clones representing the specific insertion element of M. paratuberculosis (IS900)-encoding genes and their gene products were identified and characterized. Well-defined recombinant antigens and/or epitopes representing M. paratuberculosis may facilitate the development of specific diagnostic tests and the investigation of their role in these chronic diseases.

Citing Articles

Expression cloning of gamma interferon-inducing antigens of Mycobacterium avium subsp. paratuberculosis.

Nagata R, Muneta Y, Yoshihara K, Yokomizo Y, Mori Y Infect Immun. 2005; 73(6):3778-82.

PMID: 15908411 PMC: 1111817. DOI: 10.1128/IAI.73.6.3778-3782.2005.

Characterization of novel coding sequences specific to Mycobacterium avium subsp. paratuberculosis: implications for diagnosis of Johne's Disease.

Paustian M, Amonsin A, Kapur V, Bannantine J J Clin Microbiol. 2004; 42(6):2675-81.

PMID: 15184451 PMC: 427819. DOI: 10.1128/JCM.42.6.2675-2681.2004.

Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp. paratuberculosis.

Bannantine J, Hansen J, Paustian M, Amonsin A, Li L, Stabel J J Clin Microbiol. 2004; 42(1):106-14.

PMID: 14715739 PMC: 321684. DOI: 10.1128/JCM.42.1.106-114.2004.

Seroreactivities against Saccharomyces cerevisiae and Mycobacterium avium subsp. paratuberculosis p35 and p36 antigens in Crohn's disease patients.

Shafran I, Piromalli C, Decker J, Sandoval J, Naser S Dig Dis Sci. 2002; 47(9):2079-81.

PMID: 12353858 DOI: 10.1023/a:1019685131692.

Mycobacterium avium subsp. paratuberculosis in Veterinary Medicine.

Harris N, Barletta R Clin Microbiol Rev. 2001; 14(3):489-512.

PMID: 11432810 PMC: 88986. DOI: 10.1128/CMR.14.3.489-512.2001.

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