Functional Interaction Between BLM Helicase and 53BP1 in a Chk1-mediated Pathway During S-phase Arrest

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2004 Sep 15

PMID 15364958

Citations 67

Authors

Sagar Sengupta

Ana I Robles

Steven P Linke

Natasha I Sinogeeva

Ran Zhang

Remy Pedeux

Irene M Ward

Arkady Celeste

Andre Nussenzweig

Junjie Chen

Thanos D Halazonetis

Curtis C Harris

Affiliations

Soon will be listed here.

Abstract

Bloom's syndrome is a rare autosomal recessive genetic disorder characterized by chromosomal aberrations, genetic instability, and cancer predisposition, all of which may be the result of abnormal signal transduction during DNA damage recognition. Here, we show that BLM is an intermediate responder to stalled DNA replication forks. BLM colocalized and physically interacted with the DNA damage response proteins 53BP1 and H2AX. Although BLM facilitated physical interaction between p53 and 53BP1, 53BP1 was required for efficient accumulation of both BLM and p53 at the sites of stalled replication. The accumulation of BLM/53BP1 foci and the physical interaction between them was independent of gamma-H2AX. The active Chk1 kinase was essential for both the accurate focal colocalization of 53BP1 with BLM and the consequent stabilization of BLM. Once the ATR/Chk1- and 53BP1-mediated signal from replicational stress is received, BLM functions in multiple downstream repair processes, thereby fulfilling its role as a caretaker tumor suppressor.

Citing Articles

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