Monocyte Chemoattractant Protein-1 (MCP-1) Expression in Human Articular Cartilage. Induction by Peptide Regulatory Factors and Differential Effects of Dexamethasone and Retinoic Acid

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1992 Aug 1

PMID 1365641

Citations 41

Authors

P M Villiger

R Terkeltaub

M Lotz

Affiliations

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Abstract

Monocyte influx and activation in synovial joints are important in the pathogenesis of both degenerative and inflammatory arthropathies. In this study, we demonstrate the potential of articular cartilage to directly modulate these events. IL-1-stimulated human articular chondrocytes transcribed 0.7-kb monocyte chemoattractant protein-1 (MCP-1) mRNA. In situ hybridization of cartilage organ cultures revealed MCP-1 transcripts in chondrocytes in the superficial tangential zone within 2 h of stimulation with IL-1. Chondrocytes in deeper layers responded by 4 h and reached maximum MCP-1 mRNA levels by 8-12 h. IL-1-stimulated cartilage organ and chondrocyte monolayer cultures released functional monocyte chemotactic activity. This was neutralized by a monoclonal antibody specific for MCP-1, and was associated with the synthesis and secretion of immunoreactive 13-kD and 15-kD isoforms of MCP-1. Regulators and signal transduction pathways involved with the expression of the MCP-1 gene in chondrocytes were analyzed. Steady-state mRNA levels were increased by the known chondrocyte activators IL-1, tumor necrosis factor alpha, LPS, platelet-derived growth factor, and transforming growth factor beta. In addition, leukemia inhibitory factor induced MCP-1 gene expression and protein synthesis, identifying this cytokine as a new regulator of chondrocyte function. Dexamethasone blunted the induction of MCP-1 gene expression by IL-1 and by activators of protein kinase A as well as protein kinase C signal transduction pathways. In contrast, retinoic acid strongly increased phorbol myristate acetate-induced MCP-1 expression and potentiated the effects of IL-1 and LPS. In conclusion, chondrocytes express MCP-1 in response to factors that are present in cartilage or synovium. This provides a mechanism by which cartilage can play an active role in the initiation and progression of arthritis.

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References

Peracchia F, Ferrari G, Poggi A, Rotilio D . IL-1 beta-induced expression of PDGF-AA isoform in rabbit articular chondrocytes is modulated by TGF-beta 1. Exp Cell Res. 1991; 193(1):208-12. DOI: 10.1016/0014-4827(91)90557-b. View

Villiger P, Cronin M, Amenomori T, Wachsman W, Lotz M . IL-6 production by human T lymphocytes. Expression in HTLV-1-infected but not in normal T cells. J Immunol. 1991; 146(2):550-9. View

Zachariae C, Anderson A, Thompson H, Appella E, Mantovani A, Oppenheim J . Properties of monocyte chemotactic and activating factor (MCAF) purified from a human fibrosarcoma cell line. J Exp Med. 1990; 171(6):2177-82. PMC: 2187957. DOI: 10.1084/jem.171.6.2177. View

Nicholson R, Mader S, Nagpal S, Leid M, Rochette-Egly C, Chambon P . Negative regulation of the rat stromelysin gene promoter by retinoic acid is mediated by an AP1 binding site. EMBO J. 1990; 9(13):4443-54. PMC: 552237. DOI: 10.1002/j.1460-2075.1990.tb07895.x. View

Leonard E, Yoshimura T . Human monocyte chemoattractant protein-1 (MCP-1). Immunol Today. 1990; 11(3):97-101. DOI: 10.1016/0167-5699(90)90035-8. View

Palladino M, Morris R, Starnes H, Levinson A . The transforming growth factor-betas. A new family of immunoregulatory molecules. Ann N Y Acad Sci. 1990; 593:181-7. DOI: 10.1111/j.1749-6632.1990.tb16110.x. View

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

Giguere V, Ong E, Evans R, Tabin C . Spatial and temporal expression of the retinoic acid receptor in the regenerating amphibian limb. Nature. 1989; 337(6207):566-9. DOI: 10.1038/337566a0. View

Wahl S, Hunt D, Wakefield L, McCartney-Francis N, Wahl L, Roberts A . Transforming growth factor type beta induces monocyte chemotaxis and growth factor production. Proc Natl Acad Sci U S A. 1987; 84(16):5788-92. PMC: 298948. DOI: 10.1073/pnas.84.16.5788. View

10.

Furutani Y, Nomura H, Notake M, Oyamada Y, Fukui T, Yamada M . Cloning and sequencing of the cDNA for human monocyte chemotactic and activating factor (MCAF). Biochem Biophys Res Commun. 1989; 159(1):249-55. DOI: 10.1016/0006-291x(89)92430-3. View

11.

Matsushima K, Larsen C, DuBois G, Oppenheim J . Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J Exp Med. 1989; 169(4):1485-90. PMC: 2189236. DOI: 10.1084/jem.169.4.1485. View

12.

Pujol J, LOYAU G . Interleukin-1 and osteoarthritis. Life Sci. 1987; 41(10):1187-98. DOI: 10.1016/0024-3205(87)90196-2. View

13.

Villiger P, Lotz M . Differential expression of TGF beta isoforms by human articular chondrocytes in response to growth factors. J Cell Physiol. 1992; 151(2):318-25. DOI: 10.1002/jcp.1041510213. View

14.

Schule R, Rangarajan P, Yang N, Kliewer S, Ransone L, Bolado J . Retinoic acid is a negative regulator of AP-1-responsive genes. Proc Natl Acad Sci U S A. 1991; 88(14):6092-6. PMC: 52028. DOI: 10.1073/pnas.88.14.6092. View

15.

Brandes M, Mai U, Ohura K, Wahl S . Type I transforming growth factor-beta receptors on neutrophils mediate chemotaxis to transforming growth factor-beta. J Immunol. 1991; 147(5):1600-6. View

16.

Louthrenoo W, Sieck M, Clayburne G, Rothfuss S, Schumacher Jr H . Supravital staining of cells in noninflammatory synovial fluids: analysis of the effect of crystals on cell populations. J Rheumatol. 1991; 18(3):409-13. View

17.

Lotz M, Guerne P . Interleukin-6 induces the synthesis of tissue inhibitor of metalloproteinases-1/erythroid potentiating activity (TIMP-1/EPA). J Biol Chem. 1991; 266(4):2017-20. View

18.

Hilton D, Gough N . Leukemia inhibitory factor: a biological perspective. J Cell Biochem. 1991; 46(1):21-6. DOI: 10.1002/jcb.240460105. View

19.

Oppenheim J, Zachariae C, Mukaida N, Matsushima K . Properties of the novel proinflammatory supergene "intercrine" cytokine family. Annu Rev Immunol. 1991; 9:617-48. DOI: 10.1146/annurev.iy.09.040191.003153. View

20.

Guerne P, Lotz M . Interleukin-6 and transforming growth factor-beta synergistically stimulate chondrosarcoma cell proliferation. J Cell Physiol. 1991; 149(1):117-24. DOI: 10.1002/jcp.1041490115. View