Development of Plasmid Cloning Vectors for Thermus Thermophilus HB8: Expression of a Heterologous, Plasmid-borne Kanamycin Nucleotidyltransferase Gene

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1992 Jan 1

PMID 1311546

Citations 17

Authors

M W Mather

J A Fee

Affiliations

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Abstract

While several Thermus genes have been cloned and T. thermophilus has been shown to be transformable, molecular genetic studies of these thermophiles have been hampered by the absence of selectable cloning vectors. We have constructed a selectable plasmid by random insertion of a heterologous gene encoding a thermostable kanamycin nucleotidyltransferase activity into a cryptic, multicopy plasmid from T. thermophilus HB8. This plasmid should serve as a suitable starting point for the development of a gene expression system for T. thermophilus.

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